Cell-cell contact and cAMP regulate the expression of a UDP glucose pyrophosphorylase gene of Dictyostelium discoideum.
Cell-cell contact and cAMP regulate the expression of a UDP glucose pyrophosphorylase gene of Dictyostelium discoideum.
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细胞间接触和 cAMP 调节盘基网柄菌 UDP 葡萄糖焦磷酸化酶基因的表达。
DOI：
10.1016/0012-1606(86)90178-8
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发表时间：
1986
影响因子：
2.7
通讯作者：
R. Dottin
中科院分区：
文献类型：
--
作者：
B. Haribabu;A. Rajkovic;R. Dottin
UDP葡萄糖焦磷酸化酶(UDPGP)(EC.2.7.7.9)是盘基网柄菌的一种发育调节酶。UDPGP的两个多肽是从Dictyostelium的mRNA翻译而来的。最近，我们克隆了一个编码UDPGP多肽的cDNA克隆(B.R.Fishel，J.A.Ragheb，A.Rajkovic，B.Haribabu，C.W.Schweinfest和R.P.Dottin(1985))。Biol.110,369-381)。通过体外翻译和免疫沉淀法检测细胞间接触和cAMP对UDPgp表达调控的影响。鼻涕虫的解聚导致UDPGP mRNA的快速丢失。将cAMP添加到这些细胞中会导致UDPGP mRNA水平的增加，尽管程度不同于正常发育时的程度。在体外观察到的两个UDPGP多肽是协同调节的。未聚集的细胞在悬浮液中饥饿和快速摇动，没有显示UDPGP mRNA的积聚。然而，向这些细胞中添加cAMP可引起UDPGP的诱导，这表明添加cAMP可以部分绕过细胞-细胞接触的要求。
UDP glucose pyrophosphorylase (UDPGP) (EC.2.7.7.9) is a developmentally regulated enzyme ofDictyostelium discoideum. Two polypeptides of UDPGP are translated fromDictyosteliummRNA. Recently we isolated a cDNA clone which encodes one of the UDPGP polypeptides (B. R. Fishel, J. A. Ragheb, A. Rajkovic, B. Haribabu, C. W. Schweinfest, and R. P. Dottin (1985).Dev. Biol.110,369–381). By hybridization with the cDNA and byin vitrotranslation and immunoprecipitation, we examined the effect of cell-cell contact and cAMP on the regulation of UDPGP expression. Disaggregation of slugs resulted in a rapid loss of UDPGP mRNA. Addition of cAMP to these cells resulted in increased levels of UDPGP mRNA, though not to the same extent as seen during normal development. The two UDPGP polypeptides observedin vitroare coordinately regulated. Unaggregated cells, starved and shaken rapidly in suspension, did not show UDPGP mRNA accumulation. However, addition of cAMP to these cells caused UDPGP induction, suggesting that the requirement for cell-cell contact could be bypassed in part by cAMP addition.
与盘基网柄菌的 UDP-葡萄糖焦磷酸化酶 mRNA 互补的 cDNA 的分子克隆。
影响因子：
2.7
作者：
Fishel,BR;Ragheb,JA;Rajkovic,A;Haribabu,B;Schweinfest,CW;Dottin,RP
通讯作者：
Dottin,RP
盘基网柄菌多种形式的UDP葡萄糖焦磷酸化酶的发育调控。
影响因子：
2.7
作者：
Fishel,BR;Manrow,RE;Dottin,RP
通讯作者：
Dottin,RP