Characterization and application of bioactive glass and chitosan nanoparticles for tooth enamel remineralization
Characterization and application of bioactive glass and chitosan nanoparticles for tooth enamel remineralization
复制标题
生物活性玻璃和壳聚糖纳米粒子在牙釉质再矿化中的表征及应用
DOI：
10.1166/jbt.2021.2703
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发表时间：
2021
影响因子：
0.1
通讯作者：
Masahiro Iijima
中科院分区：
文献类型：
--
作者：
Rina Ishikawa;Ryota Nagasaki;Naohiko Kawamura N;Enrique Ezra Zuniga Heredia;Takashi Nezu;Shuichi Ito;Hideki Aita;Masahiro Iijima
我们通过应用生物活性玻璃(BG)和壳聚糖纳米颗粒(CNPS)来研究脱矿牙釉质表面的再矿化。BG和CNPS在人工唾液中浸泡7天。用X射线衍射仪(X射线衍射仪)和扫描电子显微镜(SEM)对产物进行了分析。将牙釉质块浸泡在脱矿液中，分成5组：(1)不加任何材料；(2)含NaF的牙膏；(3)CNP水凝胶；(4)含BG的蒸馏水浆；(5)含BG的CNP水凝胶(BG-CNP)。釉质块在人工唾液中浸泡1个月，每种材料每天涂抹两次。在浸泡期间对牙釉质表面进行纳米压痕测试。我们发现，经BG和BG-CNP处理的人工唾液中形成了单向的棒状晶体，显示了羟基磷灰石的多个X射线衍射峰。脱矿液浸泡后牙釉质表面的力学性能明显下降，经BG-CNP和BG处理1个月后，牙釉质表面的力学性能明显恢复。用NaF、BG和CNP-BG浸泡唾液后，在多孔脱矿牙釉质表面填充再矿化层。因此，每天使用CNP-BG或BG有助于牙釉质的再矿化。
We investigated the remineralization of demineralized enamel surfaces through the application of bioactive glass (BG) and chitosan nanoparticles (CNPs). BG and CNPs were immersed in artificial saliva for 7 days. The resulting crystals were analyzed by X-ray diffraction (XRD) and scanning electron microscopy (SEM). Embedded enamel blocks were immersed in demineralization solution and then classified into five treatment groups: (1) No material applied; (2) toothpaste containing NaF (F); (3) CNP hydrogel; (4) distilled water slurry containing BG; and (5) CNP hydrogel containing BG (BG-CNP). The enamel blocks were immersed in an artificial saliva solution for 1 month; each material was applied twice per day. Nanoindentation testing of enamel surfaces was performed during the immersion period. We found that unidirectional rod-shaped crystals formed in the artificial saliva solutions treated with BG and BG-CNP, showing multiple XRD peaks for hydroxyapatite. The mechanical properties of enamel surfaces decreased markedly following immersion in the demineralization solution, and significantly recovered after 1 month of BG-CNP and BG treatment compared to other the specimens. Porous demineralized enamel surfaces were filled with a remineralization layer after immersion in saliva and application of NaF, BG, and CNP-BG. Thus, daily application of CNP-BG or BG facilitates enamel remineralization.