Development of a genetic selection for catalytic antibodies.
Development of a genetic selection for catalytic antibodies.
复制标题
开发催化抗体的遗传选择。
DOI：
10.1016/s0960-894x(02)00198-1
复制
发表时间：
2002
影响因子：
2.7
通讯作者：
Schultz,PeterG
中科院分区：
文献类型：
--
作者：
Gildersleeve,Jeff;Janes,Jeff;Ulrich,Helle;Yang,Priscilla;Barbas,Carlos;Schultz,PeterG
描述了一种新的用于进化具有醛缩酶活性的催化抗体的遗传选择系统的设计和评价。通过一系列的模型选择，我们已经确定了催化活性抗体的表达对大肠杆菌具有生长优势的选择条件。此外，我们提供的证据表明，增长优势是催化活性的直接结果。
The design and evaluation of a new genetic selection system for evolving catalytic antibodies with aldolase activity are described. Through a series of model selections, we have identified selection conditions where expression of a catalytically active antibody confers a growth advantage to Escherichia coli. In addition, we provide evidence that the growth advantage is a direct result of catalytic activity.