Microbial Community Structures and Important Associations Between Soil Nutrients and the Responses of Specific Taxa to Rice-Frog Cultivation
Microbial Community Structures and Important Associations Between Soil Nutrients and the Responses of Specific Taxa to Rice-Frog Cultivation
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DOI：
10.3389/fmicb.2019.01752
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发表时间：
2019-08
影响因子：
5.2
通讯作者：
Xiaomei Yi;Kai-Fang Yi;Kaikai Fang;Hui Gao;W. Dai;Linkui Cao
中科院分区：
文献类型：
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作者：
Xiaomei Yi;Kai-Fang Yi;Kaikai Fang;Hui Gao;W. Dai;Linkui Cao
稻蛙共作是我国传统的农业生产方式，近年来又被重新引入我国。水稻根际土壤中的微生物群落因参与土壤功能过程而备受关注。在这项研究中，基于Illumina MiSeq测序的技术被用来调查土壤微生物群落和功能基因模式，通过常规水稻栽培（CR）和水稻-青蛙栽培（RF）获得的样品。结果表明，RF显著影响水稻根际微生物群落组成和丰富度，表明虎蛙的引入对水稻根际微生物产生了一定的影响。结果表明，RF富集了Sandaracinaceae、Anaerolineaceae、Nitrososphaera、Nitrosotalea、Nitrosoarchaeum的操作分类单位（OTU）以及广古菌门和伞菌门的一些未分类OTU。非生物参数土壤有机碳（SOC），硝态氮（NO3--N），有效磷（AP）在RF处理下发生变化，并在建立土壤细菌，古细菌和真菌组成中发挥重要作用。利用网络分析法描述了环境因子与微生物群落的相关性。SOC与Anaerolineaceae，Methanosaeta和Scutellinia密切相关。NO3−-N与Opitutus、Geelus和Methanosaeta呈显著正相关。NH_4 ~+-N与氧化亚铁盐呈显著正相关，TN与硝化纤维素呈显著正相关。与常规CR相比，RF极大地富集了特定的微生物类群。这些类群可能参与复杂有机质的分解和土壤养分的转化，从而通过改善养分循环促进植物生长。土壤剖面中微生物分类和功能组成的独特模式表明这些水稻土中存在功能冗余。RF可以通过改变SOC和AP水平来显著影响细菌、古菌和真菌群落。
Rice-frog cultivation is a traditional farming system in China and has been reintroduced as an agricultural practice in China in recent years. The microbial community in paddy rhizospheric soils has attracted much attention because many microorganisms participate in functional processes in soils. In this study, Illumina MiSeq sequencing-based techniques were used to investigate soil microbial communities and functional gene patterns across samples obtained by conventional rice cultivation (CR) and rice-frog cultivation (RF). The results showed that RF significantly affected the microbial community composition and richness, which indicated that the rhizospheric microorganisms responded to the introduction of tiger frogs into the paddy fields. Operational taxonomic units (OTUs) from Sandaracinaceae, Anaerolineaceae, Candidatus Nitrososphaera, Candidatus Nitrosotalea, Candidatus Nitrosoarchaeum and some unclassified OTUs from Euryarchaeota and Agaricomycetes were significantly enriched by RF. The abiotic parameters soil organic carbon (SOC), nitrate nitrogen (NO3−-N), and available phosphorus (AP) changed under RF treatment and played essential roles in establishing the soil bacterial, archaeal, and fungal compositions. Correlations between environmental factors and microbial communities were described using network analysis. SOC was strongly correlated with Anaerolineaceae, Methanosaeta, and Scutellinia. NO3−-N showed strong positive correlations with Opitutus, Geobacter, and Methanosaeta. NH4+-N was strongly positively associated with Sideroxydans, and TN was strongly positively correlated with Candidatus Nitrotoga. Compared to conventional CR, RF greatly enriched specific microbial taxa. These taxa may be involved in the decomposition of complex organic matter and the transformation of soil nutrients, thus promoting plant growth by improving nutrient cycling. The unique patterns of microbial taxonomic and functional composition in soil profiles suggested functional redundancy in these paddy soils. RF could significantly affect the bacterial, archaeal, and fungal communities though changing SOC and AP levels.