Role of calcium on TPA-induced secretion of ACTH and PGE2 by pituitary cells: effect of dexamethasone.
Role of calcium on TPA-induced secretion of ACTH and PGE2 by pituitary cells: effect of dexamethasone.
复制标题
钙对 TPA 诱导垂体细胞分泌 ACTH 和 PGE2 的作用：地塞米松的作用。
DOI：
10.1016/0006-291x(86)90283-4
复制
发表时间：
1986
影响因子：
3.1
通讯作者：
M. Bouton
中科院分区：
文献类型：
--
作者：
E. Dartois;M. Bouton
在原代培养的垂体细胞中，TPA可在Ca ~（2+）存在下同时刺激ACTH和PGE ~（2+）的分泌。然而，虽然PGE 2的分泌是在Ca 2+的绝对需求下，ACTH的分泌不是。这两种分泌都被地塞米松抑制，但程度不同：PGE 2释放在地塞米松存在下被消除，而只有35%的TPA诱导的ACTH释放对地塞米松敏感。类似的抑制作用，观察与美帕林，PLA 2抑制剂，这表明PLA 2激活可能与这些分泌过程。由于地塞米松对PLA 2的抑制作用被认为是通过脂皮质素介导的，这些结果表明，垂体细胞中存在脂皮质素样蛋白，并可能参与TPA诱导的PGE 2和ACTH分泌。
In pituitary cells in primary culture, ACTH and PGE2secretions can be simultaneously stimulated by TPA, in the presence of Ca2+. However, whilst PGE2secretion is under an absolute requirement for Ca2+, ACTH secretion is not. Both secretions are inhibited by dexamethasone but to various extents: PGE2release is abolished in the presence of dexamethasone whilst only 35% of the TPA-induced ACTH release is sensitive to dexamethasone. Similar inhibitory effects are observed with mepacrine, a PLA2-inhibitor, suggesting that PLA2-activation could be related to these secretory processes. Since PLA2-inhibition by dexamethasone is claimed to be mediated via lipocortin, these results suggest that a lipocortin-like protein is present in pituitary cells and could be involved in the TPA-induced secretions of PGE2and ACTH.