Effect of Acupuncture on the Notch Signaling Pathway in Rats with Brain Injury
Effect of Acupuncture on the Notch Signaling Pathway in Rats with Brain Injury
复制标题
DOI：
10.1007/s11655-015-1969-9
复制
发表时间：
2018-07
影响因子：
2.9
通讯作者：
Yi-min Zhang;Sheng-xin Chen;Qiu-Fu Dai;Shu Jiang;Ai-lian Chen;C. Tang;Yu-qing Zhang
中科院分区：
文献类型：
--
作者：
Yi-min Zhang;Sheng-xin Chen;Qiu-Fu Dai;Shu Jiang;Ai-lian Chen;C. Tang;Yu-qing Zhang
目的观察针刺对创伤性脑损伤大鼠Notch信号通路的影响，探讨针刺干预创伤性脑损伤的机制。方法采用Feeney‘s自由落体硬膜外冲击法建立大鼠创伤性脑损伤模型，随机分为正常组、假手术组、模型组和针刺组。针刺大鼠百会、水沟、风府、崖门、合谷穴，经风府针刺崖门。然后将每组大鼠按治疗时间随机分为3个亚组，即3d亚组、7d亚组和14d亚组。采用改良神经严重度评分法(MNSS)进行神经行为评分，评价损伤程度。采用苏木精-伊红染色方法观察各组大鼠脑组织的病理变化。采用实时荧光定量聚合酶链式反应技术检测损伤侧大脑皮质Notch1、Hes1、Hes5基因表达水平的变化。结果造模后1d，模型组和针刺组mNSS评分明显高于正常组和假手术组(P<；0.01)，正常组与假手术组比较差异无统计学意义。随着治疗时间的延长，各项评分均呈下降趋势，针刺组较模型组下降更明显(P<；0.01)。HE染色病理检查显示，针刺组和模型组大鼠脑组织有较明显的变化。针刺组Notch1基因表达水平显著高于其他各组(P<；0.01)，Hes1、Hes5基因表达水平也高于其他各组(P<；0.01)。Notch1和Hes1蛋白的表达变化与mRNA的表达变化一致。各实验组mNss评分、HE染色病理结果与mRNA结果一致。结论针刺能显著促进创伤性脑损伤大鼠脑组织Notch1、Hes1、Hes5的高表达。因此，针刺可能是诱导内源性干细胞增殖、促进神经修复的重要干预手段。
ObjectiveTo observe the effect of acupuncture on the Notch signaling pathway in rats with traumatic brain injury and to explore the pathogenesis of acupuncture intervention on traumatic brain injury.MethodsFeeney’s freefall epidural impact method was used to establish a traumatic brain injury model in rats; the rats were randomly divided into a normal group, sham operation group, model group and acupuncture group. Acupuncture was performed in the Baihui (DU 20), Shuigou (DU 26), Fengfu (DU 16), Yamen (DU 15) and Hegu (LI 4) acupoints in the rat, and Yamen was punctured via Fengfu. Then, the rats in each group were randomly divided into three subgroups, namely the day 3 subgroup, day 7 subgroup and day 14 subgroup according to treatment duration. The modified neurological severity scores (mNss) method was used to perform neurobehavioral scoring for evaluating the degree of injury in the rats. The hematoxylin-eosin (HE) staining method was used to observe the pathological change in the brain tissue of rats in each group. Real-time fluorescent quantitative polymerase chain reaction (Q-PCR) technology was used to detect changes in the Notch1, Hes1 and Hes5 gene expression levels in the cortex on the injured side. Western blot was used to detect the protein expression changes.ResultsOne day after modeling, the mNss scores in the model group and in the acupuncture group were significantly higher than those in the normal and sham operation groups (P<0.01) ; there was no statistically significant difference between the normal group and the sham operation group. The scores decreased with increased treatment time, and the scores in the acupuncture group decreased more significantly than those in the model group (P<0.01). The pathological examination by the HE staining method demonstrated that the brain tissue of the rats in the acupuncture and model groups relatively significantly changed. The Notch1 gene expression level in the acupuncture group was significantly higher than the level in all of the other groups (P<0.01) ; the Hes1 and Hes5 gene expression levels were also higher in the acupuncture group. The expression changes of the Notch1 and Hes1 protein were consistent with that of mRNA. In each experimental group, the mNss score and the pathological results by the HE staining method were consistent with the mRNA results.ConclusionAcupuncture could significantly promote high expression levels of Notch1, Hes1 and Hes5 in the brain tissue of traumatic brain injury rats. Therefore, acupuncture might be an important intervention for inducing endogenous stem cell proliferation and for promoting nerve repair.