MUC6 APOMUCIN SHOWS A DISTINCT NORMAL TISSUE DISTRIBUTION THAT CORRELATES WITH LEWIS ANTIGEN EXPRESSION IN THE HUMAN STOMACH
MUC6 APOMUCIN SHOWS A DISTINCT NORMAL TISSUE DISTRIBUTION THAT CORRELATES WITH LEWIS ANTIGEN EXPRESSION IN THE HUMAN STOMACH
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DOI：
10.1016/0016-5085(95)90379-8
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发表时间：
1995-09-01
期刊：
影响因子：
29.4
通讯作者：
REAL, FX
中科院分区：
文献类型：
--
作者：
DEBOLOS, C;GARRIDO, M;REAL, FX
背景与目的：在目前已鉴定的人粘蛋白互补DNA中，有两个（MUC 5AC和MUCG）是从胃文库中克隆的。本研究检测了MUCG在正常组织中的分布，并将其与MUC 5AC以及刘易斯血型抗原的表达进行了比较。研究方法：在酶联免疫吸附试验和免疫组织化学试验中使用检测MUC 5AC和MUCG重复序列内表位的亲和纯化兔抗体。通过原位杂交分析RNA表达。免疫荧光双标记法检测apomucin和刘易斯抗原的共表达。结果：MUC 6在胃、结肠、胆囊和宫颈管中均有表达。观察到两种染色模式，核周和弥漫性细胞质，可能反映了MUCG糖基化的差异。使用免疫组化和原位杂交的胃组织切片，MUCG主要表达在胃窦粘液细胞，而MUC 5AC主要在浅表上皮和颈部腺体检测。在胃窦粘膜中，MUC 6+细胞表达刘易斯（y），而MUC 5AC+细胞表达刘易斯（B）和唾液酸-刘易斯（a）。结论：结果表明，MUCG在正常胃组织中的分布与MUC 1-MUC 5不同，MUC 5 AC和MUCG在正常胃组织中的表达不同，MUC 5 AC+和MUC 6+细胞表达不同的刘易斯抗原。
Background & Aims: Among the human mucin complementary DNAs thus far identified, two (MUC5AC and MUCG) were cloned from stomach libraries. This study examines the distribution of MUCG in normal tissues and compares it with that of MUC5AC as well as with the expression of Lewis blood group antigens. Methods: Affinity-purified rabbit antibodies detecting epitopes within the repetitive sequence of MUC5AC and MUCG were used in enzyme-linked immunosorbent assays and immunohistochemical assays. RNA expression was analyzed by in situ hybridization. Double-labeling immunofluorescence was used to study apomucin and Lewis antigen coexpression. Results: MUC6 is detected in the stomach, colon, gallbladder, and endocervix. Two patterns of staining are observed, perinuclear and diffuse cytoplasmic, possibly reflecting differences in MUCG glycosylation. Using both immunohistochemical assays and in situ hybridization on stomach tissue sections, MUCG is expressed mainly in antral mucous cells, whereas MUC5AC is detected mainly in the superficial epithelium and neck glands. In antral mucosa, MUC6+ cells express Lewis(y), whereas MUC5AC+ cells express Lewis(b) and sialyl-Lewis(a). Conclusions: It was concluded that MUCG has a distinct tissue distribution pattern, different from that of MUC1-MUC5; MUC5AC and MUCG are expressed by different cellular populations in normal stomach; and in this tissue, MUC5AC+ cells and MUC6+ cells show different patterns of Lewis antigen expression.