Comparative Transcriptomic Analysis of Virulence Factors in Leptosphaeria maculans during Compatible and Incompatible Interactions with Canola.
Comparative Transcriptomic Analysis of Virulence Factors in Leptosphaeria maculans during Compatible and Incompatible Interactions with Canola.
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DOI：
10.3389/fpls.2016.01784
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发表时间：
2016
影响因子：
5.6
通讯作者：
Bélanger RR
中科院分区：
文献类型：
--
作者：
Sonah H;Zhang X;Deshmukh RK;Borhan MH;Fernando WG;Bélanger RR
黄斑细螺旋体是一种半生物营养真菌，引起油菜黑腿病，这是油菜最具破坏性的疾病之一。在目前的研究中，为了理解和定义控制真菌的生物营养和坏死阶段的致病基因，以及那些区分相容和不相容相互作用的致病基因，进行了L. maculans的转录组分析。为此，本研究对易感品种Topas-DH16516或抗性渗入系Topas-Rlm2接种后的4个不同时间点的斑点乳杆菌分离物D5进行了比较RNA-seq分析。对16亿个Illumina reads的分析很容易识别出被候选分泌效应蛋白、CAZymes和其他致病基因过度代表的差异表达基因。通过比较相容和不相容的相互作用，鉴定出28种效应蛋白，这些蛋白的表达时间和水平表明它们在植物生物营养的建立和维持中起作用。这些包括分离物D5的所有已知的Avr基因以及8个新鉴定的效应子。此外，另外15种效应蛋白被发现在真菌的坏死阶段只表达，这支持了L. maculans在每个阶段都有一个单独的、独特的武器库的概念。至于CAZymes，它们在3 dpi时通常是高表达的，但在亲和性互作和不亲和性互作之间的表达没有差异，这表明需要其他因素来决定互作的结果。然而，在兼容相互作用中，它们在11 dpi处的显著高表达证实了它们对真菌的坏死性阶段有贡献。一个明显的例外是LysM基因，其高表达在7 dpi时在易感宿主上被观察到。在7 dpi和11 dpi时，TFs在易感Topas上的高表达支持了其在调节黄斑乳杆菌不同致病阶段相关基因中的重要作用。总之，通过比较maculans在亲和性和非亲和性相互作用中的转录组，鉴定出了关键的致病基因，这些基因不仅调节了相互作用的命运，而且还调节了真菌的生活方式转变。
Leptosphaeria maculans is a hemibiotrophic fungus that causes blackleg of canola (Brassica napus), one of the most devastating diseases of this crop. In the present study, transcriptome profiling of L. maculans was performed in an effort to understand and define the pathogenicity genes that govern both the biotrophic and the necrotrophic phase of the fungus, as well as those that separate a compatible from an incompatible interaction. For this purpose, comparative RNA-seq analyses were performed on L. maculans isolate D5 at four different time points following inoculation on susceptible cultivar Topas-DH16516 or resistant introgression line Topas-Rlm2. Analysis of 1.6 billion Illumina reads readily identified differentially expressed genes that were over represented by candidate secretory effector proteins, CAZymes, and other pathogenicity genes. Comparisons between the compatible and incompatible interactions led to the identification of 28 effector proteins whose chronology and level of expression suggested a role in the establishment and maintenance of biotrophy with the plant. These included all known Avr genes of isolate D5 along with eight newly characterized effectors. In addition, another 15 effector proteins were found to be exclusively expressed during the necrotrophic phase of the fungus, which supports the concept that L. maculans has a separate and distinct arsenal contributing to each phase. As for CAZymes, they were often highly expressed at 3 dpi but with no difference in expression between the compatible and incompatible interactions, indicating that other factors were necessary to determine the outcome of the interaction. However, their significantly higher expression at 11 dpi in the compatible interaction confirmed that they contributed to the necrotrophic phase of the fungus. A notable exception was LysM genes whose high expression was singularly observed on the susceptible host at 7 dpi. In the case of TFs, their higher expression at 7 and 11 dpi on susceptible Topas support an important role in regulating the genes involved in the different pathogenic phases of L. maculans. In conclusion, comparison of the transcriptome of L. maculans during compatible and incompatible interactions has led to the identification of key pathogenicity genes that regulate not only the fate of the interaction but also lifestyle transitions of the fungus.
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影响因子：
5.4
作者：
Ghanbarnia, Kaveh;Lydiate, Derek J.;Fernando, W. G. Dilantha
通讯作者：
Fernando, W. G. Dilantha
影响因子：
3.7
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3.5
作者：
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通讯作者：
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影响因子：
4.9
作者：
Ghanbarnia, Kaveh;Fudal, Isabelle;Borhan, M. Hossein
通讯作者：
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