Immunoassay for human serum hepcidin
Immunoassay for human serum hepcidin
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DOI：
10.1182/blood-2008-02-139915
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发表时间：
2008-11-15
期刊：
影响因子：
20.3
通讯作者：
Westerman, Mark
中科院分区：
文献类型：
--
作者：
Ganz, Tomas;Olbina, Gordana;Westerman, Mark
我们开发并验证了第一个血清酶联免疫吸附测定铁调素，主要的铁调节激素，一直很难测量。在健康志愿者中，铁调素浓度的5%至95%范围在男性中为29至254 ng/mL（n = 65），在女性中为17至286 ng/mL（n = 49），中位浓度为112 vs 65（P < .001）。检测下限为5 ng/mL。24例健康受试者的血清hepcidin浓度与其尿hepcidin浓度相关良好（r = 0.82）。血清铁调素与血清铁蛋白（r = 0.63），反映了这两种蛋白质的铁储存的调节。健康志愿者显示，与上午8点相比，中午和晚上8点血清铁调素的昼夜增加，以及对铁摄入的响应的血清铁调素的短暂升高。在与铁紊乱相关的各种临床病症中观察到铁调素水平的预期改变。缺铁性贫血（铁蛋白< 10 ng/mL）、铁缺乏性HFE血色病和青少年血色病患者的血清铁调素浓度不可检测或较低。血清铁调素浓度在患有炎症（C-反应蛋白> 10 mg/dL）、多发性骨髓瘤或慢性肾病的患者中较高。新的血清铁调素酶联免疫吸附试验产生准确和可重复的测量，适当地反映生理，病理和遗传的影响，并提供有关铁障碍的病因信息。（血。2008; 112：4292-4297）
We developed and validated the first serum enzyme-linked immunosorbent assay for hepcidin, the principal iron-regulatory hormone that has been very difficult to measure. In healthy volunteers, the 5% to 95% range of hepcidin concentrations was 29 to 254 ng/mL in men (n = 65) and 17 to 286 ng/mL in women (n = 49), with median concentrations 112 versus 65 (P < .001). The lower limit of detection was 5 ng/mL. Serum hepcidin concentrations in 24 healthy subjects correlated well with their urinary hepcidin (r = 0.82). Serum hepcidin appropriately correlated with serum ferritin (r = 0.63), reflecting the regulation of both proteins by iron stores. Healthy volunteers showed a diurnal increase of serum hepcidin at noon and 8 pm compared with 8 am, and a transient rise of serum hepcidin in response to iron ingestion. Expected alterations in hepcidin levels were observed in a variety of clinical conditions associated with iron disturbances. Serum hepcidin concentrations were undetectable or low in patients with iron deficiency anemia (ferritin < 10 ng/mL), iron-depleted HFE hemochromatosis, and juvenile hemochromatosis. Serum hepcidin concentrations were high in patients with inflammation (C-reactive protein > 10 mg/dL), multiple myeloma, or chronic kidney disease. The new serum hepcidin enzyme-linked immunosorbent assay yields accurate and reproducible measurements that appropriately reflect physiologic, pathologic, and genetic influences, and is informative about the etiology of iron disorders. (Blood. 2008; 112: 4292-4297)