mda-5, but not RIG-I, is a common target for paramyxovirus V proteins
mda-5, but not RIG-I, is a common target for paramyxovirus V proteins
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DOI：
10.1016/j.virol.2006.09.023
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发表时间：
2007-03-01
期刊：
影响因子：
3.7
通讯作者：
Goodbourn, Stephen
中科院分区：
文献类型：
--
作者：
Childs, Kay;Stock, Nicola;Goodbourn, Stephen
副粘病毒 PIV5（以前称为 SV5）对 IFN-β 的诱导受到针对细胞 RNA 解旋酶 mda-5 的病毒 V 蛋白的作用的限制。在这里，我们发现其他 12 种副粘病毒也通过其 V 蛋白保守的富含半胱氨酸的 C 末端与 mda-5 的解旋酶结构域之间的直接相互作用来靶向 mda-5。对 IFN-β 诱导的抑制不受物种限制，在一系列哺乳动物细胞以及鸟类细胞中都观察到，并且我们表明对 mda-5 功能的抑制也不限于哺乳动物细胞。相反，V蛋白不结合相关的RNA解旋酶RIG-I并且不抑制其活性。讨论了 mda-5 和 RIG-I 对 IFN-β 诱导的相对贡献。 (c) 2006 Elsevier Inc. 保留所有权利。
The induction of IFN-beta by the paramyxovirus PIV5 (formerly known as SV5) is limited by the action of the viral V protein that targets the cellular RNA helicase mda-5. Here we show that 12 other paramyxoviruses also target mda-5 by a direct interaction between the conserved cysteine-rich C-terminus of their V proteins and the helicase domain of mda-5. The inhibition of IFN-beta induction is not species-restricted, being observed in a range of mammalian cells as well as in avian cells, and we show that the inhibition of mda-5 function is also not restricted to mammalian cells. In contrast, the V proteins do not bind to the related RNA helicase RIG-I and do not inhibit its activity. The relative contributions of mda-5 and RIG-I to IFN-beta induction are discussed. (c) 2006 Elsevier Inc. All rights reserved.