Self-assembled cellular microarrays patterned using DNA barcodes
Self-assembled cellular microarrays patterned using DNA barcodes
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DOI：
10.1039/b708666k
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发表时间：
2007-01-01
期刊：
影响因子：
6.1
通讯作者：
Francis, Matthew B.
中科院分区：
文献类型：
--
作者：
Douglas, Erik S.;Chandra, Ravi A.;Francis, Matthew B.
要成功地将活细胞整合到合成装置中，需要对细胞模式进行精确的控制。在这里，我们描述了一个多功能平台，可以实现这一目标，通过DNA杂交。外源性细胞表面DNA链功能化的活细胞与打印在玻片上的同源DNA序列结合。通过这些“细胞粘附条形码”的附着是快速和特异性的，在几分钟内形成紧密排列的细胞阵列。该系统的生物物理特性被表征，该技术被用于形成具有单细胞系宽度和自组装细胞微阵列的复杂细胞图案。DNA定向细胞结合的主要优点包括能够固定贴壁和非贴壁细胞，选择性地从混合群体中捕获细胞，调整细胞的结合特性，以及重复使用广泛可用的DNA打印技术制备的底物。
The successful integration of living cells into synthetic devices requires precise control over cell patterning. Here we describe a versatile platform that can accomplish this goal through DNA hybridization. Living cells functionalized with exogenous cell-surface DNA strands bind to cognate sequences of DNA printed on glass slides. Attachment via these "cell-adhesion barcodes'' is rapid and specific, with close-packed arrays of cells forming within minutes. The biophysical properties of the system are characterized, and the technique is used to form complex cellular patterns with single-cell line widths and self-assembled cellular microarrays. Key advantages of DNA-directed cell binding include the ability to immobilize both adherent and non-adherent cells, to capture cells selectively from a mixed population, to tune the binding properties of the cells, and to reuse substrates prepared with widely available DNA printing technologies.