Multilocus sequence typing of Cryptococcus neoformans in non-HIV associated cryptococcosis in Nagasaki, Japan
Multilocus sequence typing of Cryptococcus neoformans in non-HIV associated cryptococcosis in Nagasaki, Japan
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DOI：
10.3109/13693786.2012.708883
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发表时间：
2013-04-01
期刊：
影响因子：
2.9
通讯作者：
Kohno, Shigeru
中科院分区：
文献类型：
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作者：
Mihara, Tomo;Izumikawa, Koichi;Kohno, Shigeru
隐球菌病主要由两种隐球菌引起，即新生隐球菌和加蒂隐球菌属。两者都包括几个遗传上不同的亚组，可以使用不同的分子菌株分型方法进行区分。由于对日本新城疫杆菌/盖蒂弧菌复合体的分子流行病学知之甚少，我们对来自日本长崎的35株新生弧菌和来自泰国的10株环境分离株进行了分子流行病学分析。采用URA5-限制性片段长度多态(RFLP)和多位点序列分型(MLST)进行分析。所有分离株的组合序列数据用邻接法进行评估。均为A型和交配型MATα。经URA5-RFLP方法鉴定，35株临床分离株中有32株为VNI型，其余3株为VNII型。其中31株为MLST序列类型(ST)5，1株为ST32，3株为ST43。所有环境分离株均为分子型VNI，其中4株为MLST ST5，6株为ST4。我们的研究表明，长崎的新生隐孢子虫分离株在基因上是同源的，大部分分离株是ST5。
Cryptococcosis is primarily caused by two Cryptococcus species, i.e., Cryptococcus neoformans and C. gattii. Both include several genetically diverse subgroups that can be differentiated using various molecular strain typing methods. Since little is known about the molecular epidemiology of the C. neoformans/C. gattii species complex in Japan, we conducted a molecular epidemiological analysis of 35 C. neoformans isolates from non-HIV patients in Nagasaki, Japan and 10 environmental isolates from Thailand. All were analyzed using URA5-restriction fragment length polymorphism (RFLP) and multilocus sequence typing (MLST). Combined sequence data for all isolates were evaluated with the neighbor-joining method. All were found to be serotype A and mating type MAT alpha. Thirty-two of the 35 clinical isolates molecular type VNI, while the three remaining isolates were VNII as determined through the URA5-RFLP method. Thirty-one of the VNI isolates were identified as MLST sequence type (ST) 5, the remaining one was ST 32 and the three VNII isolates were found to be ST 43. All the environmental isolates were identified as molecular type VNI (four MLST ST 5 and six ST 4). Our study shows that C. neoformans isolates in Nagasaki are genetically homogeneous, with most of the isolates being ST 5.