GPCR production in a novel yeast strain that makes cholesterol-like sterols.
GPCR production in a novel yeast strain that makes cholesterol-like sterols.
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一种新型酵母菌株中 GPCR 的产生，可产生类胆固醇甾醇。
DOI：
10.1016/j.ymeth.2011.09.023
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Fraser,NiallJ
中科院分区：
文献类型：
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作者：
Kitson,SusanM;Mullen,William;Cogdell,RichardJ;Bill,RoslynM;Fraser,NiallJ
许多哺乳动物膜蛋白包括G蛋白偶联受体的活性是胆固醇依赖性的。与高等真核生物不同，酵母不制造胆固醇。相反，它们产生一种相关的分子，称为麦角固醇。由于胆固醇和麦角固醇在生物学上是不等价的，酵母作为宿主过量生产哺乳动物膜蛋白的潜力从未完全实现。为了解决这个问题，我们正试图设计一种新的酿酒酵母菌株，其中哺乳动物细胞的胆固醇生物合成途径已完全重建。到目前为止，我们已经创建了一个修改过的菌株，使胆固醇样甾醇，具有增加的能力，使G蛋白偶联受体相比，控制酵母。
The activities of many mammalian membrane proteins including G-protein coupled receptors are cholesterol-dependent. Unlike higher eukaryotes, yeast do not make cholesterol. Rather they make a related molecule called ergosterol. As cholesterol and ergosterol are biologically non-equivalent, the potential of yeast as hosts for overproducing mammalian membrane proteins has never been fully realised. To address this problem, we are trying to engineer a novel strain of Saccharomyces cerevisiae in which the cholesterol biosynthetic pathway of mammalian cells has been fully reconstituted. Thus far, we have created a modified strain that makes cholesterol-like sterols which has an increased capacity to make G-protein coupled receptors compared to control yeast.