Particle-packed column versus silica-based monolithic column for liquid chromatography-electrospray-linear ion trap-tandem mass spectrometry multiallergen trace analysis in foods
Particle-packed column versus silica-based monolithic column for liquid chromatography-electrospray-linear ion trap-tandem mass spectrometry multiallergen trace analysis in foods
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DOI：
10.1016/j.chroma.2010.10.037
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发表时间：
2010-11-26
影响因子：
4.1
通讯作者：
Mangia, Alessandro
中科院分区：
文献类型：
--
作者：
Bignardi, Chiara;Elviri, Lisa;Mangia, Alessandro
碳酸氢盐缓冲液提取法同时分析5种坚果过敏原（Ana o 2腰果Cor a 9榛子Pru 1杏仁Ara h3/4花生壶r 4核桃）在谷物和饼干中使用液相色谱-电喷雾-线性离子阱-串联质谱开发并验证了LC-ESI-LIT-MS 2。该方法基于我们早期发表的LC-MS 2-MS。的方法，旨在通过使用选择性生物标志物肽A C18颗粒填充柱和硅胶柱来鉴定和测定食品中的隐藏过敏原。在MS 2选择反应监测下，对C18整体柱进行了色谱性能的比较，结果表明，C18颗粒填充柱的色谱性能优于C18整体柱，并进一步用于方法的开发和验证。SRM）采集模式线性检测限（LOD）和定量准确度和精密度进行了评价，早餐样品富含混合的五种坚果。获得良好的线性矩阵匹配校准曲线，检测限值一般在14至55 mg坚果/kg矩阵之间变化。回收率在76 +/- 4%至94 +/- 3%范围内，RSD
A bicarbonate buffer-based extraction method for the simultaneous analysis of five nut allergens (Ana o 2 cashew-nut Cor a 9 hazelnut Pru 1 almond Ara h3/4 peanut Jug r 4 walnut) in cereals and biscuits using liquid chromatography-electrospray-linear ion trap-tandem mass spectrometry (LC-ESI-LIT-MS2) was developed and validated The method was based on our earlier published LC-MS2-based method in a research frame aimed at the identification and determination of hidden allergens in foods by using selective biomarker peptides A C18 particle-packed column and a silica-based C18 monolithic column were compared in terms of chromatographic performances such as peak shape resolution analysis time and selectivity The C18 particle-packed column exhibited better performances and was further used for method development and validation By operating under MS2 selected reaction monitoring (SRM) acquisition mode linearity limits of detection (LOD) and quantitation trueness and precision were evaluated on breakfast samples enriched with a mix of the five nuts Good linearity of the matrix matched-calibration curves was obtained and detection limit values generally varied from 14 to 55 mg nut/kg matrix Recoveries were in the 76 +/- 4% to 94 +/- 3% range with RSD