Comparative study on secretome and transmembranome of immature and mature metacestodes of Echinococcus multilocularis
Comparative study on secretome and transmembranome of immature and mature metacestodes of Echinococcus multilocularis
复制标题
多房棘球蚴未成熟和成熟后绦虫分泌组和跨膜组的比较研究
DOI：
10.1016/j.vetpar.2017.05.006
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发表时间：
2017
影响因子：
2.6
通讯作者：
Oku Y
中科院分区：
文献类型：
--
作者：
Huang F;Dang;Z;Zhang H;Yagi K;Kim KS;Joseph M;Pu W;Oku Y
泡状棘球蚴病（AE）是一种由大肠杆菌引起的世界性人畜共患病。多房性。人类通过口服虫卵而被感染。主持人E.多房性寄生虫产生免疫反应，有助于排斥和/或限制这种寄生虫的生长，作为反应，寄生虫产生抵抗这种免疫攻击的分子。本研究通过比较 4 周和 16 周的后绦虫的基因表达，确定了寄生虫感染早期感染阶段和慢性阶段的候选关键分子。首先，从 4 周和 16 周的大肠杆菌后绦虫中分离出 RNA。多房性（根室株）。此后，使用 TopHat 将长度为 50 bp 或更长的干净读数与参考基因组进行比较。 E. 转录本的功能注释。使用多步生物信息学工具对多房性进行了研究。在基因本体（GO）水平上，356和1774跨膜（TM）预测了大肠杆菌的蛋白质。多房性分别被映射为增强的“水解酶活性”和增加的“跨膜转运蛋白活性”。此外，比较 4 周和 16 周的后绦虫的基因表达水平发现了 168 个不同的表达 (DE) 基因。本研究证明，预测的 ES 和 TM 蛋白在 E 中的表达水平。多房性变化是从一个阶段到另一个阶段的转变。在未成熟或成熟的后绦虫中高度表达的基因可以作为诊断抗原和疫苗靶标的新候选基因进行探索。
Alveolar echinococcosis (AE) is a worldwide zoonosis caused byE. multilocularis. Humans become infected through oral ingestion of the eggs. Host ofE. multilocularisproduces immune responses that help to either reject and/or limit the growth of this parasite, and in response the parasite produces molecules against this immune attack. This study identifies candidate key molecules in the early infection phase and the chronic stage of the parasite infestation, through comparison of gene expression of 4- and 16-week metacestodes. First, RNA was isolated from 4- and 16-weeks metacestodes ofE. multilocularis(Nemuro strain). Thereafter, clean reads with lengths of 50 bp or longer were compared against a reference genome using TopHat. Functional annotation of transcripts ofE. multiloculariswere investigated using multi-step bioinformatics tools. At the gene ontology (GO) level, 356 and 1774 transmembrane (TM) predicted proteins of theE. multiloculariswere mapped to an enhanced ‘hydrolase activity’ and increased ‘transmembrane transporter activity’, respectively. In addition, comparison of gene expression level between 4- and 16-week metacestode revealed 168 different expression (DE) genes. This study has demonstrated that, the expression levels of predicted ES and TM proteins inE. multilocularischange in the transformation from one stage to another. Genes that are highly expressed in immature or mature metacestode could be explored as novel candidates for diagnostic antigens and vaccine targets.