HYDROXYL RADICAL FORMATION IN HUMAN GASTRIC-JUICE
HYDROXYL RADICAL FORMATION IN HUMAN GASTRIC-JUICE
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DOI：
10.1111/j.1440-1746.1992.tb01027.x
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发表时间：
1992-09-01
影响因子：
4.1
通讯作者：
BALASUBRAMANIAN, KA
中科院分区：
文献类型：
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作者：
NALINI, S;RAMAKRISHNA, BS;BALASUBRAMANIAN, KA
羟基自由基是由氧产生的最有效的自由基，与胃十二指肠黏膜的损伤有关。本文对54例经内镜检查胃十二指肠黏膜正常的成人和39例慢性十二指肠溃疡患者的胃液产生羟基自由基的能力进行了研究。羟基自由基的产生是通过二甲基亚砜生成甲醛来测定的。与其他体液不同的是，该反应可以在不需要过氧化氢（H2O2）、抗坏血酸和铁等外部催化剂的情况下进行。对H2O2、铁和抗坏血酸的测定表明，这些催化剂已经存在于胃液中。正常受试者与十二指肠溃疡患者胃液中这些成分的浓度均无显著差异，但十二指肠溃疡患者的H2O2含量略高。虽然自由基的产生已经在其他体液中进行了研究，但这是第一次报道在正常人体胃液中产生这些活性物质的病例。由于羟基的产生在十二指肠溃疡中没有显著增强，我们建议将注意力转向这种疾病的粘膜抗氧化防御。
The hydroxyl radical is the most potent free radical derived from oxygen, and has been implicated in damage caused to the gastroduodenal mucosa. The ability of human gastric juice to generate hydroxyl radicals has been investigated in 54 adults with endoscopically normal gastroduodenal mucosa and in 39 patients with chronic duodenal ulcer. Hydroxyl radical production was measured by the formation of formaldehyde from dimethylsulfoxide. Unlike other body fluids, this reaction could proceed without the extraneous addition of catalysts such as hydrogen peroxide (H2O2), ascorbate and iron. Measurement of H2O2, iron and ascorbate showed that these catalysts are already present in the gastric juice. There was no significant difference in the concentration of these components in gastric juice between normal subjects and patients with duodenal ulcer, except that H2O2 levels were slightly higher in duodenal ulcer patients. Although generation of free radicals has been investigated in other body fluids, this is the first reported case regarding the production of these active species in normal human gastric juice. Since hydroxyl production is not significantly enhanced in duodenal ulcer, we suggest that attention may be turned to mucosal antioxidant defences in this disease.