Developmental competence of in vitro matured porcine oocytes after electrical activation
Developmental competence of in vitro matured porcine oocytes after electrical activation
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DOI：
10.1262/jrd.48.271
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发表时间：
2002-06-01
影响因子：
1.8
通讯作者：
Nagashima, H
中科院分区：
文献类型：
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作者：
Kurihara, T;Kurome, M;Nagashima, H
比较了TCM 199和NCSU 23体外成熟猪卵母细胞经电激活后的发育能力。在以TCM 199为基础的IVM系统中使用的颗粒-卵丘-卵母细胞复合物（GCOCs）和以NCSU 23为基础的IVM系统中使用的卵丘-卵母细胞复合物（COCs）的核相观察中，大多数GCOCs是完全生长的核团细胞停滞卵母细胞（GV I，68.6%），另一方面，只有33.8%的COCs处于GV I期。两种体外成熟系统获得的卵母细胞的体外成熟率（93.2%，82/88 vs 91.3%，63/69）、正常卵裂率（70.7%，58/82 vs 65.1%，41/63）和囊胚发育率（37.8%，31/82 vs 34.9%，22/63）均无显著差异。然而，NCSU 23体外培养系统获得的孤雌胚泡细胞数高于TCM 199体外培养系统获得的孤雌胚泡细胞数（35.6 ± 4.5 vs 22.6 ± 2.0; P
Developmental competence of in vitro matured porcine oocytes obtained by TCM199- and NCSU 23-based IVM systems after electrical activation were compared. When the nuclear phase of granulosa-cumulus-oocyte complexes (GCOCs) used in the TCM199-based IVM system and cumulus-oocyte complexes (COCs)used in the NCSU 23-based IVM system were observed, most of GCOCs were fully grown dictyate arrest oocytes (GV I, 68.6%), on the other hand, only 33.8% of COCs were at GV I stage. There were no differences in the rates of maturation (93.2%, 82/88 vs 91.3%, 63/69), normal cleavage (70.7%, 58/82 vs 65.1%, 41/63) and development to blastocysts (37.8%, 31/82 vs 34.9%, 22/63) between the in vitro matured oocytes obtained by the two IVM systems. However, the parthenogenetic blastocysts derived from the NCSU23-based IVM system had higher cell numbers than the blastocysts derived from the TCM199-based IVM system (35.6+/-4.5 vs 22.6+/-2.0; P