LIPOPEROXIDATIVE DAMAGE ON LEAD-EXPOSURE IN RAT-BRAIN AND ITS IMPLICATIONS ON MEMBRANE-BOUND ENZYMES
LIPOPEROXIDATIVE DAMAGE ON LEAD-EXPOSURE IN RAT-BRAIN AND ITS IMPLICATIONS ON MEMBRANE-BOUND ENZYMES
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DOI：
10.1111/j.1600-0773.1994.tb01077.x
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发表时间：
1994-02-01
期刊：
影响因子：
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通讯作者：
GILL, KD
中科院分区：
文献类型：
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作者：
SANDHIR, R;JULKA, D;GILL, KD
我们研究了铅暴露对大脑不同区域脂质过氧化的影响，脂质过氧化是一种膜的退化过程。铅处理（50 mg/kg b.wt.灌胃）给大鼠8周，导致铅在大脑所有区域的显著积累，在海马体中最大。铅暴露后脂质过氧化作用增强，且脂质过氧化作用的增强与铅水平的升高呈线性相关（r = 0.75）。抗氧化酶超氧化物歧化酶，过氧化氢酶和谷胱甘肽过氧化物酶的活性方面的神经细胞的抗氧化能力减少。铅处理也改变了谷胱甘肽的状态，即还原型谷胱甘肽的水平降低，伴随着氧化型谷胱甘肽的积累。此外，谷胱甘肽还原酶的活性显着降低铅治疗的动物。铅暴露后，膜结合酶乙酰胆碱酯酶活性明显降低，脂质过氧化反应的增加与乙酰胆碱酯酶活性的降低呈直线相关（r = -0.83）。结果提示铅可能通过过氧化损伤膜发挥其神经毒性作用。
We have investigated the effect of lead exposure on lipid peroxidation, a deteriorative process of the membranes, in the different regions of the brain. Lead treatment (50 mg/kg b.wt. intragastrically) for a period of eight weeks to rats resulted in a significant accumulation of lead in all the regions of brain, at maximum in hippocampus. The lipid peroxidation was accentuated following lead exposure and there was a linear correlation between the increase in lipid peroxidation and increase in lead levels (r = 0.75). The antioxidant capacity of the neuronal cells in terms of the activity of antioxidant enzymes superoxide dismutase, catalase and glutathione peroxidase was diminished. Lead treatment also altered the glutathione status i.e. levels of reduced glutathione were lowered, accompanied with the accumulation of oxidized glutathione. Furthermore, the activity of glutathione reductase was significantly lowered in lead-treated animals. The activity of membrane bound enzyme acetylcholinesterase was significantly inhibited following lead exposure and there was a linear correlation between the increase in lipid peroxidation and decrease in acetylcholinesterase activity (r = -0.83). It appears from the results that lead may exert its neurotoxic effects via peroxidative damage to the mebranes.