Smartphone-read phage lateral flow assay for point-of-care detection of infection
Smartphone-read phage lateral flow assay for point-of-care detection of infection
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DOI：
10.1039/d2an01499h
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发表时间：
2023-01-05
期刊：
影响因子：
4.2
通讯作者：
Kourentzi，Katerina
中科院分区：
文献类型：
--
作者：
Chabi，Maede;Vu，Binh;Kourentzi，Katerina
COVID-19 大流行凸显了迫切需要敏感、负担得起且广泛可及的护理点检测。在这里，我们展示了一种使用与抗体和 HRP 酶缀合的 M13 噬菌体的新型通用 LFA 平台技术，该技术可在复杂的临床基质中提供高分析灵敏度和出色的性能。我们还报告了其完全集成到基于化学发光的敏感智能手机可读侧流分析中，用于检测 SARS-CoV-2 核蛋白。我们在噬菌体 LFA 中筛选了 84 个抗核蛋白单克隆抗体对，并使用 FluorChem 凝胶记录系统鉴定了鼻拭子提取物中核蛋白 LoD 为 25 pg mL−1 的抗体对，并通过内部开发的智能手机阅读器对测试进行成像和分析时得出 100 pg mL−1 的 LoD。智能手机读取的测试阳性临床样本（N = 15，已知 Ct）的 LFA 信号与阴性临床样本（N = 11）的信号存在统计差异（p < 0.001）。噬菌体 LFA 技术与智能手机化学发光成像相结合，可以及时开发针对 SARS-CoV-2 及其他疾病的超灵敏、经济实惠的即时检测平台。
The COVID-19 pandemic has highlighted the urgent need for sensitive, affordable, and widely accessible testing at the point of care. Here we demonstrate a new, universal LFA platform technology using M13 phage conjugated with antibodies and HRP enzymes that offers high analytical sensitivity and excellent performance in a complex clinical matrix. We also report its complete integration into a sensitive chemiluminescence-based smartphone-readable lateral flow assay for the detection of SARS-CoV-2 nucleoprotein. We screened 84 anti-nucleoprotein monoclonal antibody pairs in phage LFA and identified an antibody pair that gave an LoD of 25 pg mL−1 nucleoprotein in nasal swab extract using a FluorChem gel documentation system and 100 pg mL−1 when the test was imaged and analyzed by an in-house-developed smartphone reader. The smartphone-read LFA signals for positive clinical samples tested (N = 15, with known Ct) were statistically different (p < 0.001) from signals for negative clinical samples (N = 11). The phage LFA technology combined with smartphone chemiluminescence imaging can enable the timely development of ultrasensitive, affordable point-of-care testing platforms for SARS-CoV-2 and beyond.