Activities of enzymes that metabolize platelet-activating factor (1-Alkyl-2-acetyl-sn-glycero-3-phosphocholine) in neutrophils and eosinophils from humans and the effect of a calcium ionophore.
Activities of enzymes that metabolize platelet-activating factor (1-Alkyl-2-acetyl-sn-glycero-3-phosphocholine) in neutrophils and eosinophils from humans and the effect of a calcium ionophore.
复制标题
人类中性粒细胞和嗜酸性粒细胞中血小板激活因子（1-烷基-2-乙酰基-sn-甘油-3-磷酸胆碱）代谢酶的活性以及钙离子载体的作用。
DOI：
10.1016/0006-291x(82)90928-7
复制
发表时间：
1982
影响因子：
3.1
通讯作者：
Snyder,F
中科院分区：
文献类型：
--
作者：
Lee,TC;Malone,B;Wasserman,SI;Fitzgerald,V;Snyder,F
描述了人体血细胞中具有降压、血小板聚集和炎症特性的生物活性磷脂(1-alkyl-2-acetyl-sn-glycero-3-phosphocholine)的激活和失活的酶系统。结果证明，在人类中性粒细胞和嗜酸性粒细胞中存在烷基二羟基丙酮-磷酸合成酶(在脂质中形成O-烷基连接)、1-烷基-2-裂解-sn-甘油-3-磷胆碱：乙酰胆碱：乙酰基-CoA乙酰基转移酶(产生生物活性分子)和1-烷基-2-乙酰基-sn-甘油-3-磷胆碱：乙酰胆碱：乙酰水解酶(破坏生物活性)。用离子载体A23187处理正常中性粒细胞后，乙酰基转移酶和乙酰水解酶活性均增加数倍，但烷基二羟基丙酮-磷酸合酶活性不受离子载体的影响。从嗜酸性粒细胞增多症患者分离的嗜酸性粒细胞比从正常人分离的嗜酸性粒细胞具有显著更高的所有研究酶的活性。我们的结果表明，负责1-烷基-2-乙酰基-sn-甘油-3-磷胆碱合成的乙酰基转移酶可能在释放这种生物活性磷脂的人体血细胞中发挥重要作用。此外，发现钙离子流量对乙酰转移酶活性有很大的影响。
Enzymatic systems in human blood cells are described for the activation and inactivation of a biologically active phospholipid (1-alkyl-2-acetyl-sn-glycero-3-phosphocholine) with hypotensive, platelet-aggregating, and inflammatory properties. The results document the presence of alkyldihydroxyacetone-phosphate synthase (forms the O-alkyl linkage in lipids), 1-alkyl-2-lyso-sn-glycero-3-phosphocholine: acetyl-CoA acetyltransferase (produces the biologically active molecule), and 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine: acetylhydrolase (destroys the biological activity) in human neutrophils and eosinophils. Both the acetyltransferase and acetylhydrolase activities are increased severalfold after treatment of normal neutrophils with ionophore A23187; however, alkyldihydroxyacetone-phosphate synthase activity is not influenced by the ionophore. Eosinophils isolated from patients with eosinophilia have significantly greater activities of all the enzymes studied than the eosinophils isolated from normal individuals. Our results indicate the acetyltransferase responsible for 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine synthesis may serve an important role in human blood cells that release this biologically active phospholipid. Moreover, the acetyltransferase activity was found to be dramatically influenced by calcium flux.