Production of poly(3-hydroxyalkanoates) containing aromatic substituents by Pseudomonas oleovorans
Production of poly(3-hydroxyalkanoates) containing aromatic substituents by Pseudomonas oleovorans
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DOI：
10.1021/ma951185a
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发表时间：
1996-02-26
期刊：
影响因子：
5.5
通讯作者：
Fuller, RC
中科院分区：
文献类型：
--
作者：
Curley, JM;Hazer, B;Fuller, RC
以5-(4‘-甲苯基)戊酸、5-(4’-乙基苯基)戊酸、5-(4‘-联苯基)戊酸和8-(4’-甲苯基)辛酸为唯一碳源或以5-苯基戊酸和5-甲苯基戊酸为唯一碳源，分别培养了油酸假单胞菌。对于聚合物生产，5-(4‘-甲苯基)戊酸是五种最有效的生长底物。它导致了聚-3-羟基-5-(4‘-甲苯基)戊酸酯的产生，这是一种玻璃化转变温度为18摄氏度，熔融转变温度为95摄氏度的结晶聚合物。这种聚(3-羟基烷酸酯)(PHA)显然是第一个含有芳香族结晶性细菌PHA的例子。当5-苯基戊酸和5-(4‘-甲苯基)戊酸等摩尔混合物共培养时，得到的聚合物含有36mol%的3-羟基-5-苯基戊酸酯和摩尔%的3-羟基-5-(4’-甲苯基)戊酸，且不结晶。
Pseudomonas oleovorans was grown separately on 5-(4'-tolyl)valeric acid, 5-(4'-ethylphenyl)valeric acid, 5-(4'-biphenyl)valeric acid, and 8-(4'-tolyl)octanoic acid either as the sole carbon source or as a coefeed with either nonanoic acid or 5-phenylvaleric acid. For polymer production, 5-(4'-tolyl)valeric acid was the most effective growth substrate of the five. It resulted in the production of poly-3-hydroxy-5-(4'-tolyl)valerate, a crystalline polymer with a glass transition temperature of 18 degrees C and a melting transition of 95 degrees C. This poly(3-hydroxyalkanoate) (PHA) is apparently the first example of a crystalline aromatic-containing bacterial PHA. When P. oleovorans was cofed an equimolar mixture of 5-phenylvaleric acid and 5-(4'-tolyl)valeric acid, the polymer produced contained 36 mol % of 3-hydroxy-5-phenylvalerate and 64 mol % of 3-hydroxy-5-(4'-tolyl)valerate, and it did not crystallize.