Changes of Photosynthetic Behaviors and Photoprotection during Cell Transformation and Astaxanthin Accumulation in Haematococcus pluvialis Grown Outdoors in Tubular Photobioreactors.
Changes of Photosynthetic Behaviors and Photoprotection during Cell Transformation and Astaxanthin Accumulation in Haematococcus pluvialis Grown Outdoors in Tubular Photobioreactors.
复制标题
管状光生物反应器中雨生红球藻细胞转化和虾青素积累过程中光合行为和光保护的变化
DOI：
10.3390/ijms18010033
复制
发表时间：
2016-12-26
影响因子：
5.6
通讯作者：
Liu J
中科院分区：
文献类型：
--
作者：
Zhang L;Su F;Zhang C;Gong F;Liu J
室外培养的雨红球藻可诱导绿色运动细胞向非运动细胞转化，并可诱导虾青素积累。在培养的最初3 d（细胞转化阶段），光吸收和光合电子传递变得更有效。经过5 d的培养（虾青素积累期），每个活性反应中心（ABS/RC）的光吸收增加，但电子传递效率（ψo）和电子传递量子产率（φEo）随时间的增加而降低，说明虾青素积累过程中光合能利用能力明显下降，导致光合光吸收与能量利用不平衡。在强光下（例如中午），它将不可避免地加剧光抑制。然而，随着孵育时间的增加，雨水蛭的光抑制水平下降，这反映在孵育前3 d中午测定的Fv/Fm显著下降，但孵育7天后受影响很小，与黎明前测定的相比。这可能是因为非光化学猝灭、质体末端氧化酶、光系统I循环电子传递、防御酶和积累的虾青素可以保护细胞免受光抑制。
The cell transformation from green motile cells to non-motile cells and astaxanthin accumulation can be induced in the green alga Haematococcus pluvialis cultured outdoors. In the initial 3 d of incubation (cell transformation phase), light absorption and photosynthetic electron transport became more efficient. After five days of incubation (astaxanthin accumulation phase), the light absorption per active reaction center (ABS/RC) increased, but the efficiency of electron transport (ψo) and the quantum yield of electron transport (φEo) decreased with increased time, indicating that the capacity of photosynthetic energy utilization decreased significantly during astaxanthin accumulation, leading to an imbalance between photosynthetic light absorption and energy utilization. It would inevitably aggravate photoinhibition under high light, e.g., at midday. However, the level of photoinhibition in H. pluvialis decreased as the incubation time increased, which is reflected by the fact that Fv/Fm determined at midday decreased significantly in the initial 3 d of incubation, but was affected very little after seven days of incubation, compared with that determined at predawn. This might be because the non-photochemical quenching, plastid terminal oxidase, photosystem I cyclic electron transport, defensive enzymes and the accumulated astaxanthin can protect cells against photoinhibition.