LncRNA CTBP1-AS2 regulates miR-216a/PTEN to suppress ovarian cancer cell proliferation
LncRNA CTBP1-AS2 regulates miR-216a/PTEN to suppress ovarian cancer cell proliferation
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DOI：
10.1186/s13048-020-00689-6
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发表时间：
2020-07-25
影响因子：
4
通讯作者：
Zhu, Genhai
中科院分区：
文献类型：
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作者：
Cui, Kaiying;Zhu, Genhai
背景我们分析了TCGA数据集，观察了CTBP1-AS2在卵巢癌（OC）中的下调，而CTBP1-AS2的功能仅在糖尿病和心肌细胞肥大中被研究，而在癌症生物学中还没有。因此，我们分析了CTBP1-AS2在OC中的参与。结果CTBP1-AS2在OC中表达下调，提示OC患者生存率低。荧光素酶活性检测中CTBP1-AS2与miR-216a相互作用，而CTBP1-AS2和miR-216a过表达对彼此的表达无明显影响。然而，在CTBP1-AS2过表达后，观察到miR-216 a的靶点PTEN的表达水平增加。过表达miR-216a后，OC细胞的增殖率明显增加。CTBP1-AS2和PTEN过表达导致OC细胞增殖率降低，miR-216a过表达的作用减弱。结论CTBP1-AS2调控miR-216a/PTEN抑制OC细胞增殖。
Background We analyzed TCGA dataset and observed the downregulation of CTBP1-AS2 in ovarian cancer (OC), while the function of CTBP1-AS2 has only been investigated in diabetes and cardiomyocyte hypertrophy, but not in cancer biology. We therefore analyzed the involvement of CTBP1-AS2 in OC. Result We found that CTBP1-AS2 was downregulated in OC and predicted poor survival. CTBP1-AS2 in luciferase activity assay interacted with miR-216a, while overexpression of CTBP1-AS2 and miR-216a had no significant effects on the expression of each other. However, increased expression level of PTEN, a target of miR-216a, was observed after CTBP1-AS2 overexpression. Increased proliferation rate of OC cells was observed after the overexpression of miR-216a. CTBP1-AS2 and PTEN overexpression resulted in the reduced proliferation rate of OC cells and reduced effects of miR-216a overexpression. Conclusion CTBP1-AS2 regulates miR-216a/PTEN to suppress OC cell proliferation.