The uremic solute indoxyl sulfate acts as an antioxidant against superoxide anion radicals under normal-physiological conditions
The uremic solute indoxyl sulfate acts as an antioxidant against superoxide anion radicals under normal-physiological conditions
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DOI：
10.1016/j.febslet.2010.04.046
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发表时间：
2010-07-02
期刊：
影响因子：
3.5
通讯作者：
Otagiri, Masaki
中科院分区：
文献类型：
--
作者：
Miyamoto, Yohei;Iwao, Yasunori;Otagiri, Masaki
用电子顺磁共振（ESR）技术，结合2-乙氧羰基-2-甲基-3，4-二氢-2H-吡咯-1-氧化物（EMPO），研究了尿毒症溶质硫酸吲哚酚（IS）对黄嘌呤/黄嘌呤氧化酶（X/XO）体系和活化中性粒细胞产生的超氧阴离子自由基（O-2）的清除作用。研究结果表明，正常生理血清浓度的IS（0.1-10 μ M）的存在下，导致在不影响XO活性的EMPO-超氧化物加合物的形成减少。此外，IS显示出对由活化的中性粒细胞产生的细胞源性O-2（中心点-）的清除活性。此外，IS还清除羟基自由基。这些研究结果表明，IS作为一种新的内源性抗氧化剂在正常生理条件下。(C)2010年由Elsevier B. V.代表欧洲生物化学学会联合会出版。
The effect of the uremic solute indoxyl sulfate (IS) on scavenging superoxide anion radicals (O-2(center dot)) generated from both the xanthine/xanthine oxidase (X/XO) system and activated neutrophils was investigated by electron paramagnetic resonance spectroscopy, combined with 2-ethoxycarbonyl-2-methyl-3,4-dihydro-2H-pyrrole-1-oxide (EMPO). The findings show that the presence of normal-physiological serum concentrations of IS (0.1-10 mu M) resulted in decreased formation of EMPO-superoxide adduct without affecting XO activity. Furthermore, IS showed scavenging activity against cell-derived O-2(center dot-) generated from activated neutrophils. In addition, IS also eliminated hydroxyl radicals. These findings suggest that IS acts as a novel endogenous antioxidant under normal-physiological conditions. (C) 2010 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.