Genetic polymorphisms of 26 Y-STR loci in the Mongolian minority from Horqin district, China
Genetic polymorphisms of 26 Y-STR loci in the Mongolian minority from Horqin district, China
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科尔沁蒙古族26个Y-STR位点遗传多态性
DOI：
10.1007/s00414-016-1387-3
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发表时间：
2016-07-01
影响因子：
2.1
通讯作者：
Zha, Lagabaiyila
中科院分区：
文献类型：
--
作者：
Fu, Xiaoliang;Fu, Yong;Zha, Lagabaiyila
为了研究科尔沁地区蒙古族Y染色体短串联重复序列(Y-STR)的群体数据，利用商品化的DYS439、DYS448、DYS456、DYS458、DYS481、DYS533、DYS549、DYS570、DYS576、DYS635、DYS643、DYS388、DYS449、DYS460、DYGATAH4等26个Y-STR在298名蒙古族个体中进行了单倍型分析。我们还调查了10名健康男性的血迹、唾液斑点、精液斑点、毛囊、指甲和汗液潜伏指纹，以测试这种新的Y-STRS系统的直接扩增效率。计算得到的蒙古族群体DYS389I和DYS385a/b基因座的基因多样性平均值分别为0.3024~0.9510。23个Y-STR基因座(DYS19、DYS385a/b、DYS389I、DYS389II、DYS390、DYS391、DYS392、DYS393、DYS437、DYS438、DYS439、DYS448、DYS456、DYS458、DYS481、DYS533、DYS549、DYS570、DYS576、DYS635、DYS643和YGATAH4)的277个单倍型的判别能力为92.95%。通过在26Y系统中增加3个Y-STR(DYS388、DYS449和DYS460)，识别能力提高到94.63%，共观察到282个单倍型。计算了群体关系，并与Y染色体单倍型参考数据库中的7个群体和之前发表的10个亚洲群体的数据进行了比较。科尔沁地区的蒙古族人口与其他民族有很大的不同。我们的结果表明这26个Y-STR在蒙古族群体中具有高度的遗传多态，这对现有的中国民族遗传信息有很大的贡献。通过直接扩增，我们获得了所有血迹、唾液斑点、毛囊和指甲的完整轮廓；六个精液斑点；以及一个汗液潜在指纹。结果表明，26Y-STR系统是一种有价值的男性性别鉴定工具，该试剂盒适用于血迹、唾液斑点、毛囊、指甲等多种样本的直接扩增。
To study the population data of Y chromosome STR (Y-STRs) of the Mongolian minority population residing in the Horqin district, we analyzed haplotypes of 26 Y-STRs (DYS19, DYS385a/b, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS481, DYS533, DYS549, DYS570, DYS576, DYS635, DYS643, DYS388, DYS449, DYS460, and YGATAH4) in 298 unrelated Chinese Mongolian individuals using the commercially available Goldeneye® DNA ID 26Y system. We also investigated blood stains, saliva spots, semen spots, hair follicles, fingernails, and sweat latent fingerprints from ten healthy males for testing the efficiency of direct amplification of this new Y-STRs system. The calculated average gene diversity values of the Mongolian population ranged from 0.3024 to 0.9510 for the DYS389I and DYS385a/b loci, respectively. The discriminatory capacity was 92.95 % with 277 observed haplotypes using 23 Y-STR loci (DYS19, DYS385a/b, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS481, DYS533, DYS549, DYS570, DYS576, DYS635, DYS643, and YGATAH4). By adding three more Y-STRs (DYS388, DYS449, and DYS460) to the 26Y system, the discriminatory capacity was increased to 94.63 % with a total of 282 observed haplotypes. Population relationships were calculated and compared with seven populations available from the Y chromosome haplotype reference database and data from ten Asian populations published previously. The Mongolian minority population residing in Horqin district is significantly different from other populations. Our results indicated that these 26 Y-STRs were highly genetically polymorphic in the Mongolian group and this contributes greatly to existing Chinese ethnic genetic information. As a result of direct amplification, we have obtained full profile from all blood stains, saliva spots, hair follicles, and fingernails; six semen spots; and one sweat latent fingerprint. It revealed that the 26 Y-STR system was a valuable tool for male sex analysis in forensic field and the kit was highly adaptive to direct amplification of various samples including blood stain, saliva spot, hair follicle, and fingernail.