WOUND FLUID FROM CHRONIC LEG ULCERS CONTAINS ELEVATED LEVELS OF METALLOPROTEINASES MMP-2 AND MMP-9
WOUND FLUID FROM CHRONIC LEG ULCERS CONTAINS ELEVATED LEVELS OF METALLOPROTEINASES MMP-2 AND MMP-9
复制标题
DOI：
10.1111/1523-1747.ep12359590
复制
发表时间：
1993-07-01
影响因子：
6.5
通讯作者：
GRINNELL, F
中科院分区：
文献类型：
--
作者：
WYSOCKI, AB;STAIANOCOICO, L;GRINNELL, F
本研究的目的是确定金属蛋白酶水平是否在人类慢性伤口液中升高。 收集急性（乳房切除术）和慢性（腿部溃疡）伤口的血液和伤口液样本，并通过明胶酶谱法测定样本的金属蛋白酶谱。 与血清相比，急性伤口液（乳房切除术液）含有显著增加（5 - 10倍）的几种金属蛋白酶，包括72-kDa和94-kDa明胶酶（MMP-2和MMP-9）的水平。 在慢性伤口液中，不仅这些酶水平比乳房切除术液增加另外5至10倍，而且活化的酶和酶原种类似乎都存在。 我们的研究结果表明，非愈合性溃疡的发展和环境中含有高水平的活化金属蛋白酶，这可能会导致慢性组织周转和失败的伤口闭合。
The purpose of the present research was to determine if metalloproteinase levels were elevated in human chronic wound fluid. Samples of blood and wound fluid from acute (mastectomy) and chronic (leg ulcer) wounds were collected, and metalloproteinase profiles of the samples were determined by gelatin zymography. Compared to serum, acute wound fluid (mastectomy fluid) contained markedly increased levels (five- to tenfold) of several metalloproteinases including 72-kDa and 94-kDa gelatinases (MMP-2 and MMP-9). In chronic wound fluid, not only were these enzyme levels increased another five- to tenfold over mastectomy fluid, but also both activated enzyme and proenzymes species appeared to be present. Our results suggest that non-healing ulcers develop and environment containing high levels of activated metalloproteinases, which may result in chronic tissues turnover and failed wound closure.