Functional integrity of granulosa cells from polycystic ovaries
Functional integrity of granulosa cells from polycystic ovaries
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DOI：
10.1046/j.1365-2265.1996.724545.x
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发表时间：
1996-05-01
影响因子：
3.2
通讯作者：
Trounson, AO
中科院分区：
文献类型：
--
作者：
Almahbobi, G;Anderiesz, C;Trounson, AO
目的多囊卵巢综合征是一种常见的人类不孕症，是一种病因不明的部分特征性综合征。本研究的目的是描述多囊卵巢颗粒细胞的功能完整性。收集24例排卵期和7例无排卵期患者的多囊卵巢卵泡抽吸物，以及24例未治疗和10例超排卵患者的正常卵巢卵泡抽吸物，所有患者均收集卵母细胞进行体外成熟和受精。检测不同患者卵巢颗粒细胞中FSH受体和细胞凋亡的情况结果I-125标记的重组人FSH与无排卵多囊卵巢颗粒细胞的结合显著高于正常卵巢颗粒细胞(180%)和超排卵巢颗粒细胞(163%)，但与正常颗粒细胞的配体结合率无显著差异(P>0.05)，而经FSH培养的无排卵多囊卵巢颗粒细胞产生的雌二醇明显多于正常卵巢颗粒细胞，但与超排卵颗粒细胞相似(平均值+/-SEM)。分别为244.94+/-22.02，24.23+/-2.92,211.87+/-50.39nmol/L/24 h)，流式细胞仪分析显示，正常卵巢和多囊卵巢颗粒细胞存活和凋亡的比例(平均+/-SDM分别为70+/-5和7+/-1%)相似。这些数据提供了多囊卵巢的大多数卵泡不闭锁的直接证据。
OBJECTIVE The polycystic ovarian syndrome Is frequently associated with human infertility and is a partially characterized syndrome of unknown aetiology, The aim of this study was to describe the functional integrity of granulosa cells from polycystic ovaries.PATIENTS Follicular aspirates were collected from polycystic ovaries of ovulatory (n = 24) and anovulatory (n = 7) patients, Follicular aspirates were also collected from normal ovaries of untreated (n = 24) and superovulated (n = 10) subjects, All patients were enrolled for the recovery of their oocytes for in vitro maturation and fertilization.MEASUREMENTS FSH receptors and apoptosis were measured in the granulosa cells of the different patients, FSH-stimulated oestradiol and LH-stimulated progesterone production by granulosa cells of the different patients were also measured.RESULTS The binding of I-125-labelled human recombinant FSH to granulosa cells from anovulatory subjects with polycystic ovaries was significantly higher than that found in granulosa cells from normal (180%) and superovulated (163%) ovaries, However, the ligand binding to granulosa cells from ovulatory subjects with polycystic ovaries was not significantly higher than that found in normal granulosa cells, Also, granulosa cells obtained from anovulatory subjects with polycystic ovaries cultured with FSH produced more oestradiol than normal granulosa cells but oestradiol production was similar to that of granulosa cells from superovulated ovaries (mean +/- SEM, 244.94 +/- 22.02, 24.23 +/- 2.92, 211.87 +/- 50.39 nmol/l/24 h, respectively), Flow cytometric analysis showed that the proportions of viable and apoptotic granulosa cells (mean +/- SDM, 70 +/- 5 and 7 +/- 1%, respectively) were similar in normal subjects and in those with polycystic ovaries.CONCLUSION We conclude that most of the granulosa cells of polycystic ovaries are healthy and non-apoptotic, expressing high levels of FSH receptors and highly responsive to this hormone in culture. These data provide direct evidence that most of the follicles of polycystic ovaries are not atretic.