Two-Plasmid Vector System for Independently Controlled Expression of Green and Red Fluorescent Fusion Proteins in Staphylococcus aureus
Two-Plasmid Vector System for Independently Controlled Expression of Green and Red Fluorescent Fusion Proteins in Staphylococcus aureus
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DOI：
10.1128/aem.00144-13
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发表时间：
2013-05-01
影响因子：
4.4
通讯作者：
Firth, Neville
中科院分区：
文献类型：
--
作者：
Brzoska, Anthony J.;Firth, Neville
我们构建了一个系统，用于在金黄色葡萄球菌中调节绿色荧光蛋白（GFP）和红色荧光蛋白（RFP）融合体的共表达。它通过细胞分裂蛋白 FtsZ 和 Noc 的同时定位得到验证，并用于检测其天然球状宿主中肌动蛋白样 ParM 质粒分配蛋白的丝形成。
We have constructed a system for the regulated coexpression of green fluorescent protein (GFP) and red fluorescent protein (RFP) fusions in Staphylococcus aureus. It was validated by simultaneous localization of cell division proteins FtsZ and Noc and used to detect filament formation by an actin-like ParM plasmid partitioning protein in its native coccoid host.