The role of light in the regulation of anthocyanin accumulation in Gerbera hybrida
The role of light in the regulation of anthocyanin accumulation in Gerbera hybrida
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DOI：
10.1007/s10725-004-4454-6
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发表时间：
2004-11-01
影响因子：
4.2
通讯作者：
Wang, XJ
中科院分区：
文献类型：
--
作者：
Meng, XC;Xing, T;Wang, XJ
以非洲菊（Gerberahybrida）舌状花（rf）为材料，研究了光照对舌状花色素形成的影响。当第1阶段的花序在体内用铝箔覆盖时，rf花瓣的着色被强烈地阻断，CHS（查尔酮合成酶）和DFR（二氢黄酮醇-4-还原酶）的基因表达被抑制。离体培养的rfs也得到类似的结果。覆盖的植物上的叶子导致减少色素沉着相比，在体内的花序覆盖。去除绿色苞片对色素沉着没有显著影响，花色素苷浓度保持在与对照相似的水平。通过对rf花瓣质体超微结构的观察，探讨光合作用在光调控花色素形成中的可能作用。rf表皮细胞内的质体在第2阶段的花中表现出特征性的叶绿体形态，在第3阶段退化。然后在完全开放的rf花瓣中它们变成类似于染色质的结构（第6阶段）。在离体和活体遮光花序的rf花瓣质体中观察到类似的质体样结构。此外，DCMU，光合抑制剂，没有表现出显着的影响，光诱导的花青素积累。我们的数据表明，光是一个重要的因素，在非洲菊rf花瓣色素沉着和花瓣本身作为一个光传感器网站感知光信号。从不同的光质评价，蓝光促进CHS和DFR的基因表达，和红光增强CHS的基因表达，表明光感受器响应蓝光和红光参与了非洲菊花色素的光调节。
In the present work, the pigmentation regulated by light was investigated in ray floret (rf) of Gerbera hybrida. When inflorescences from stage 1 were covered with aluminium foil in vivo the pigmentation of the rf petals was strongly blocked and the gene expression of CHS ( Chalcone synthase) and DFR ( Dihydroflavonol-4-reductase) was inhibited. Similar results were obtained when the detached rfs were cultured in vitro. Covering of the leaves on the plants resulted in reduced pigmentation compared with the covering of inflorescences in vivo. Removal of the green bracts did not affect the pigmentation significantly and the anthocyanin concentration was maintained at a level similar to that of the control. The ultrastructure of the plastids in rf petals was examined to investigate the possible role of photosynthesis in light regulation of flower pigmentation. Plastids within rf epidermal cells showed a characteristic chloroplast morphology in flowers at stage 2, which deteriorated by stage 3. They then changed to a chromoplast- like structure in fully opened rf petals ( stage 6). Similar chromoplast- like structures were observed in the plastids of the rf petals from inflorescences both shaded in vivo and in vitro. Additionally, DCMU, a photosynthetic inhibitor, did not show a significant effect on light-induced anthocyanin accumulation. Our data suggest that light is an important factor for pigmentation of rf petal in Gerbera and the petal itself acts as a light sensor site to perceive the light signal. From the different light qualities evaluated, blue light promoted gene expression of CHS and DFR, and red light enhanced the gene expression of CHS, indicating the photoreceptors responding to blue and red light involved in the photoregulation of flower pigmentation in Gerbera.