Cloning and characterization of the myelin basic protein gene from mouse: one gene can encode both 14 kd and 18.5 kd MBPs by alternate use of exons
Cloning and characterization of the myelin basic protein gene from mouse: one gene can encode both 14 kd and 18.5 kd MBPs by alternate use of exons
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小鼠髓磷脂碱性蛋白基因的克隆和表征：一个基因可以通过交替使用外显子同时编码 14 kd 和 18.5 kd MBP
DOI：
10.1016/s0092-8674(85)80109-4
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发表时间：
1985
期刊：
影响因子：
64.5
通讯作者：
L. Hood
中科院分区：
文献类型：
--
作者：
N. Takahashi;A. Roach;D. Teplow;S. Prusiner;L. Hood
用大鼠小（Mr 14，000）髓鞘碱性蛋白（MBP）的cDNA克隆筛选小鼠粘粒文库。分离出完整的MBP基因。用cDNA克隆检测到编码Mr 14，000 MBP的5个外显子。用合成寡核苷酸检测到的第6个外显子编码对Mr 18，500形式MBP特异的41个氨基酸。将所有6个外显子拼接在一起将产生Mr 18，500 MBP，而省略外显子V的mRNA将被翻译产生Mr 14，000 MBP。进一步的实验表明，在小鼠基因组中可能存在单个MBP基因，并且小鼠MBP转录物存在单个主要5′端，起始甲硫氨酸密码子的47 × 5′。
A mouse cosmid library was screened with a cDNA clone for rat small (Mr14,000) myelin basic protein (MBP). The entire MBP gene was isolated. Five exons were detected with the cDNA clone, which encode the Mr14,000 MBP A sixth exon, detected using a synthetic oligonucleotide, encodes the 41 amino acids specific to the Mr18,500 form of MBP. Splicing together all six exons would give rise to Mr18,500 MBP, while an mRNA omitting exon V would be translated to produce Mr14,000 MBP Further experiments indicate that there is probably a single MBP gene in the mouse genome, and that there is a single major 5′ end for mouse MBP transcripts, 47 by 5′ of the initiator methionine codon.
使用髓磷脂碱性蛋白 cDNA 克隆表征小鼠髓磷脂碱性蛋白信使 RNA。
DOI：
10.1073/pnas.81.1.18
发表时间：
1984
影响因子：
11.1
作者：
Zeller,NK;Hunkeler,MJ;Campagnoni,AT;Sprague,J;Lazzarini,RA
通讯作者：
Lazzarini,RA