Analysing cell-free plasma DNA and SLE disease activity
Analysing cell-free plasma DNA and SLE disease activity
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DOI：
10.1111/j.1365-2362.2010.02435.x
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发表时间：
2011-06-01
影响因子：
5.5
通讯作者：
Stuhlmeier, Karl M.
中科院分区：
文献类型：
--
作者：
Atamaniuk, Johanna;Hsiao, Yu-Yang;Stuhlmeier, Karl M.
背景多年来，循环中游离DNA的论证和确认越来越被认为是一种有价值的诊断工具。同样，人们早就知道，自身抗体靶向的 DNA 结构在系统性红斑狼疮 (SLE) 中发挥着核心作用，循环中的 DNA-抗体复合物是 SLE 的标志之一。因此，本研究的目的是调查 SLE 患者游离血浆 DNA 水平的波动是否以及在多大程度上与疾病严重程度相关。方法采集 13 名 SLE 患者和 13 名健康对照者的血液，分析是否存在抗 dsDNA、抗 ssDNA、抗核小体、抗组蛋白抗体以及游离 DNA 浓度。计算每位患者的 SLE 疾病活动指数 (SLEDAI)。 结果如本文所示，与健康受试者相比，SLE 患者的血浆游离 DNA 水平显着升高，抗 dsDNA、抗 ssDNA、抗组蛋白和抗核小体抗体也显着升高。此外，SLE 患者的游离 DNA 和抗组蛋白抗体之间存在统计学上显着的相关性。然而，没有发现疾病活动性与抗 dsDNA、抗 ssDNA 和抗核小体抗体浓度之间存在相关性。令人惊讶的是，在本研究中更重要的是，游离 DNA 水平和 SLEDAI 评分之间没有相关性。结论所提供的数据似乎排除了测量游离血浆 DNA 作为评估 SLE 患者疾病活动性的廉价、简单和快速工具的可能性。需要对更大的患者群体进行进一步的研究来证实我们的结果。
P>BackgroundOver the years, the demonstration and confirmation of cell-free DNA in the circulation has increasingly been recognized as a valuable diagnostic tool. Likewise, it has been known for some time that DNA structures that are targeted by auto-antibodies play a central role in systemic lupus erythematosis (SLE) and that DNA-antibody complexes in the circulation are one of the hallmarks of SLE. Investigating whether and to what degree fluctuations in free plasma DNA levels in patients with SLE might correspond to disease severity was therefore the goal of this investigation.MethodsBlood from 13 patients with SLE and from 13 healthy controls was taken and analysed for the presence of anti-dsDNA, anti-ssDNA, anti-nucleosome, anti-histone antibodies as well as for cell-free DNA concentrations. For each patient, the SLE disease activity index (SLEDAI) was calculated.ResultsAs demonstrated herein, compared to healthy subjects, cell-free DNA plasma levels in patients with SLE were significantly increased and so were anti-dsDNA, anti-ssDNA, anti-histone and anti-nucleosome antibodies. Furthermore, a statistically significant correlation was noted between cell-free DNA and anti-histone antibodies in patients with SLE. However, no correlation was noted between disease activity and anti-dsDNA, anti-ssDNA and anti-nucleosome antibody concentrations. Surprisingly, and more important in the context of this study, there was no correlation between cell-free DNA levels and SLEDAI scores.ConclusionsThe presented data seem to exclude measuring free plasma DNA as an inexpensive, simple and quick tool to assess disease activity in patients with SLE. Further studies on a larger patient population would be needed to confirm our results.