Silver loaded biodegradable carboxymethyl chitin films with long-lasting antibacterial activity for infected wound healing
Silver loaded biodegradable carboxymethyl chitin films with long-lasting antibacterial activity for infected wound healing
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载银可生物降解羧甲基甲壳素薄膜具有持久抗菌活性，可促进感染伤口愈合
DOI：
10.1039/d2bm01046a
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Xulin Jiang
中科院分区：
文献类型：
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作者：
Siyao Lv;Xulin Jiang
细菌相关感染是人类皮肤感染的主要原因之一，与伤口愈合延迟和继发并发症有关。本文以羧甲基甲壳素为基料，在不使用交联剂的情况下，制备了一系列新型的生物降解膜。所有羧甲基甲壳素膜均具有良好的柔韧性、高透明度和适当的吸水能力，并能为伤口愈合提供湿润的环境。将银离子（Ag+）引入到LTCF-5膜上，其在羧甲基甲壳素膜中具有最好的机械强度（干态56.39 MPa，湿态0.66 MPa），并且高于已报道的生物可降解敷料和市售敷料的机械强度。与市售含银的水纤维敷料（AQUACEL®）相比，该复合膜具有良好的强度和生物降解性，可缓慢缓释Ag+，对金黄色葡萄球菌（S. aureus和E.大肠杆菌，经磷酸盐缓冲液浸泡7 d后，抑菌率几乎为100%，无明显细胞毒性。更重要的是，载银羧甲基甲壳素膜可以促进S.金黄色葡萄球菌感染的全层皮肤缺损的体内模型，因为它的持久的抗菌活性，良好的生物相容性，渗出液的吸收和保持潮湿的环境的能力。因此，载银羧甲基甲壳素膜是感染伤口愈合的优良候选者。
Bacteria-related infections are one of the main causes of human skin infections, which are associated with the delay of wound healing and secondary complications. In this work, a series of novel biodegradable films based on thermosensitive carboxymethyl chitin were prepared without using any crosslinkers. All the carboxymethyl chitin films had good flexibility, high transparency, and appropriate water absorption capacity, and could provide a moist environment for wound healing. The silver ions (Ag+) were incorporated on the LTCF-5 film, which had the best mechanical strength (56.39 MPa in the dry state and 0.66 MPa in the wet state) among the carboxymethyl chitin films and was higher than those of the reported biodegradable dressings and commercially available dressings. Compared with the commercial hydrofiber dressing with silver (AQUACEL®), the composite film could provide slow and sustained release of Ag+ with good strength and biodegradability, and displayed excellent long-lasting antibacterial activity in vitro against both S. aureus and E. coli without obvious cytotoxicity, which still possessed good antibacterial activity with almost 100% bacteriostatic rates after soaking in phosphate buffered saline for 7 days. More importantly, the Ag+ loaded carboxymethyl chitin film could promote infected cutaneous wound healing in a S. aureus infected full-thickness cutaneous defect in vivo model because of its long-lasting antibacterial activity, good biocompatibility, exudate absorption and ability to maintain a moist environment. Thus Ag+ loaded carboxymethyl chitin films are excellent candidates for infected wound healing.