Interstitial fibrosis in the heart: differences in extracellular matrix proteins and matrix metalloproteinases in end-stage dilated, ischaemic and valvular cardiomyopathy
Interstitial fibrosis in the heart: differences in extracellular matrix proteins and matrix metalloproteinases in end-stage dilated, ischaemic and valvular cardiomyopathy
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DOI：
10.1111/j.1365-2559.2006.02398.x
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发表时间：
2006-05-01
期刊：
影响因子：
6.4
通讯作者：
Schnabel, PA
中科院分区：
文献类型：
--
作者：
Herpel, E;Pritsch, M;Schnabel, PA
目的：探讨细胞外基质(ECM)蛋白和基质金属蛋白酶(MMPs)在不同心肌病所致终末期心力衰竭中的分布是否存在差异。方法与结果：39例移植心脏中，扩张型心肌病(DCM)15例，缺血性心肌病(ICM)17例，瓣膜性心肌病(VCM)7例。对来自四个不同地点的跨壁样本进行了调查。冰冻切片进行I、III、IV型胶原、层粘连蛋白、纤维连接蛋白、基质金属蛋白酶-1、-2、-9的免疫组织化学染色。测定了其体积密度。所有ECM成分在DCM中的表达频率均高于ICM。比较ICM和VCM，除III型胶原在ICM中的表达频率明显高于ICM外，VCM中所有蛋白的表达频率均高于ICM。与DCM和VCM相比，VCM的III型胶原和层粘连蛋白的体积密度显著增加。结论：ECM蛋白在DCM、ICM和VCM中的分布不同，提示它们可以从形态上区分间质纤维化，尤其是基质蛋白的表达。
Aims: To investigate whether or not there are differences in the distribution of extracellular matrix (ECM) proteins and matrix metalloproteinases (MMPs) in end-stage heart failure underlying different cardiomyopathies.Methods and results: Thirty-nine explanted human hearts were investigated: 15 with dilated cardiomyopathy (DCM), 17 with ischaemic cardiomyopathy (ICM) and seven with valvular cardiomyopathy (VCM). Transmural samples from four different sites were investigated. Frozen sections were processed for immunohistochemistry for collagens type I, III, IV, laminin and fibronectin, as well as MMP-1, -2 and -9. Volume densities were determined. All ECM components were expressed more frequently in DCM than in ICM. Comparing ICM with VCM, all proteins were found more frequently in VCM than in ICM except for type III collagen, which was significantly more frequent in ICM. Comparing DCM and VCM, VCM showed significantly higher volume densities for type III collagen and laminin. MMPs showed only slight variations between the cardiomyopathies.Conclusion: The distribution of ECM proteins differs between DCM, ICM and VCM, which suggests that they can be morphologically discriminated by interstitial fibrosis, especially by their expression of matrix proteins.