Laminin γ2-chain fragment circulating level increases in patients with metastatic pancreatic ductal cell adenocarcinomas
Laminin γ2-chain fragment circulating level increases in patients with metastatic pancreatic ductal cell adenocarcinomas
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DOI：
10.1016/j.canlet.2004.11.052
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发表时间：
2005-07-08
期刊：
影响因子：
9.7
通讯作者：
Sekiguchi, K
中科院分区：
文献类型：
--
作者：
Katayama, M;Funakoshi, A;Sekiguchi, K
Larninin-5（LN 5）仅由上皮细胞表达，并被认为在被蛋白酶切割后增强细胞迁移，导致LN 5 γ 2-链（G2 F）的N-末端片段脱落。本文测定了181例消化系统疾病患者和15例健康人的血清G_2F水平。胰腺导管细胞癌肝转移组G2 F水平明显升高，而无肝转移组G2 F水平无明显升高。良性胰腺肿瘤（胰腺囊肿和导管内乳头状粘液性肿瘤）患者的G2 F水平与健康志愿者相似。另一方面，γ 1-链的水平是几种层粘连蛋白异源三聚体的共同组成部分。还测定了这些患者循环中的γ 1-链（G1 F）的N-末端片段，仅在肝细胞癌患者中观察到G1 F水平略有增加。有趣的是，在胆管癌和胆囊癌患者以及转移性胰腺导管细胞腺癌患者中观察到循环G2 F/G1 F比值显著增加。循环G2 F水平的增加，特别是与循环G1 F水平相比，应该与浸润性癌中的LN 5过表达相关，独立于整个身体中的基底膜代谢。(c)2004爱思唯尔爱尔兰有限公司保留所有战斗。
Larninin-5 (LN5) is expressed solely by epithelial cells and considered to enhance cell migration after being cleaved by proteases, leading to the shedding of the N-terminal fragment of the LN5 gamma 2-chain (G2F). We estimated circulating G2F level in 181 patients with various digestive diseases and 15 healthy subjects. The G2F level in pancreatic ductal cell adenocarcinoma patients with liver metastases was markedly elevated, but that in patients without liver metastases was not significantly elevated. The G2F levels in patients with benign pancreatic tumours (pancreatic cysts and intraductal papillary mucinous tumours) were similar to that in healthy volunteers. On the other hand, the level of the gamma 1-chain is a common constituent of several laminin heterotrimers. The N-terminal fragment of gamma 1-chain (G1F) in the circulation of these patients was also determined, and a slight increase in G1F levels was observed only in hepatocellular carcinoma patients. Interestingly, a significant increase in circulating G2F/G1F ratio was observed in patients with bile duct and gallbladder carcinoma, as well as in those with metastatic pancreatic ductal cell adenocarcinoma. The increase in the circulating G2F level, particularly compared with circulating G IF level, should correlated with LN5 overexpression in invasive carcinomas, independently of basement membrane metabolism in the entire body. (c) 2004 Elsevier Ireland Ltd. All fights reserved.