Investigations on the relationship between sperm fertilizing ability and androgen-binding protein in the hypophysectomized, pregnenoloneenolone-injected rat.
Investigations on the relationship between sperm fertilizing ability and androgen-binding protein in the hypophysectomized, pregnenoloneenolone-injected rat.
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DOI：
10.1210/endo-114-4-1419
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发表时间：
1984-04
期刊：
影响因子：
4.8
通讯作者：
Catherine Tananis Anthony;B. J. Danzo;M. Orgebin‐Crist
中科院分区：
文献类型：
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作者：
Catherine Tananis Anthony;B. J. Danzo;M. Orgebin‐Crist
2 mg孕烯醇酮维持垂体切除大鼠血浆睾酮水平较低，但睾丸激素水平正常。该模型系统用于检验雄激素结合蛋白（ABP）在维持高腔内雄激素浓度对附睾精子受精能力的发育和维持的重要性。从术后第1天开始，给去垂体大鼠注射孕烯醇酮（1、0.5、0.2或0.02 mg/100 g BW），持续14天。假手术大鼠和去垂体大鼠组注射精油作为对照。实验结束时，测定精子受精能力、组织重量、ABP和睾酮水平。1毫克和0.5毫克的孕烯醇酮剂量导致小鼠睾丸激素水平分别为假手术动物的67%和29%。血浆睾酮水平与去垂体、注射任何剂量孕烯醇酮的油的对照组没有差异。三个最高剂量的孕烯醇酮导致睾丸ABP水平与假手术水平（2 pmol/器官）没有统计学差异。给药1 mg时，附睾ABP含量维持在假手术对照水平（30 pmol/器官）；在0.5和0.2 mg剂量下，ABP水平分别为假手术水平的76%和73%。附睾ABP特异性含量（每100 mg组织皮摩尔）在1、0.5和0.2 mg剂量下维持在假手术对照水平。使用三种最高剂量的孕烯醇酮，精子受精能力维持在假手术控制水平。0.02 mg孕烯醇酮剂量组的所有测试参数值与垂体切除、油注射对照组的值无统计学差异。ABP与精子受精率呈显著正相关。这些数据表明，孕烯醇酮单独治疗可维持垂体切除大鼠睾丸和附睾ABP水平，并且当腔内雄激素水平较低而ABP水平接近正常时，精子受精能力可以维持。这表明ABP可能在维持正常精子受精能力中起重要作用。
Hypophysectomized rats maintained with 2 mg pregnenolone have low plasma testosterone levels, but rete testis levels are normal. This model system was used to examine the importance of androgen-binding protein (ABP) in maintaining high luminal androgen concentrations for the development and maintenance of sperm fertilizing ability in the epididymis. Hypophysectomized rats were injected with pregnenolone (1, 0.5, 0.2, or 0.02 mg/100 g BW) for 14 days, starting 1 day after surgery. Sham-operated rats and a group of hypophysectomized rats were injected with oils as controls. At the end of the experimental period, sperm fertilizing ability, tissue weights, ABP, and testosterone levels were determined. The 1- and 0.5-mg doses of pregnenolone resulted in rete testis testosterone levels that were 67% and 29%, respectively, of the levels found in sham-operated animals. Plasma testosterone levels were not different from levels found in hypophysectomized, oil-injected controls with any of the pregnenolone doses. The three highest doses of pregnenolone resulted in levels of testicular ABP that were not statistically different from sham-operated levels (2 pmol/organ). Epididymal ABP content was maintained at sham-operated control levels (30 pmol/organ) with the 1-mg dose; with the 0.5- and 0.2-mg doses, ABP levels were 76% and 73% of sham-operated levels, respectively. Epididymal ABP specific content (picomoles per 100 mg tissue) was maintained at sham-operated control levels with the 1-, 0.5-, and 0.2-mg doses. Sperm fertilizing ability was maintained at sham-operated control levels with the three highest doses of pregnenolone. The 0.02-mg dose of pregnenolone resulted in values that were not statistically different from hypophysectomized, oil-injected control values for all parameters tested. A significant positive correlation existed between ABP and sperm fertilizing ability. These data demonstrate that testicular and epididymal ABP levels can be maintained in hypophysectomized rats with pregnenolone treatment alone, and that sperm fertilizing ability can be maintained when intraluminal androgen levels are low and ABP levels are near normal. This suggests that ABP may be important in the maintenance of normal sperm fertilizing ability.