Isolation and characterization of cold responsive NAC gene from Lepidium latifolium
Isolation and characterization of cold responsive NAC gene from Lepidium latifolium
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DOI：
10.1007/s11033-012-1828-0
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发表时间：
2012-10-01
影响因子：
2.8
通讯作者：
Ahmed, Zakwan
中科院分区：
文献类型：
--
作者：
Aslam, Mohammad;Grover, Atul;Ahmed, Zakwan
低温胁迫是限制作物产量的主要因素之一。生长在较冷地区的植物由于存在“耐寒基因”而适应严酷的环境。在现代农业实践中利用这些基因之前，分离和功能鉴定是很重要的。在此，我们克隆了宽叶独行草全长的NAC基因(1388个碱基)。该基因属于NAP亚群，也包括与LlaNAC最近的近亲拟南芥的ANAC056。对ANAC056的上游分析和微阵列数据分析表明，LlaNAC也可能受ABA调控。然而，定量转录表达分析表明，LlaNAC的转录在低温胁迫下上调，并在不同浓度的脱落酸、水杨酸、氯化钙和乙烯的作用下下调。还有一种可能性是，该基因可能受到一种成分尚不清楚的途径的调控。任何进一步了解LlaNAC基因表达调控机制的研究都可能在植物生物技术和作物改良中发现巨大的重要性。
Cold stress is one of the major limiting factor in crop productivity. Plants growing in colder regions acclimatize to severe conditions owing to the presence of 'cold stress tolerant genes'. Isolation and functional characterization of these genes are important before their exploitation in modern agricultural practices. Here, we have cloned full length NAC gene (1,388 bp) from Lepidium latifolium (LlaNAC). This gene belongs to NAP sub-group which also includes ANAC056 of Arabidopsis thaliana, nearest relative of LlaNAC. Upstream analysis and microarray data analysis of ANAC056 suggested that LlaNAC might also be ABA-regulated. However, quantitative transcript expression analysis revealed that LlaNAC transcript upregulated by cold stress and downregulated in response to varying concentrations of abscisic acid, salicylic acid, calcium chloride and ethylene. There is also a possibility that the gene may be getting regulated by a pathway whose components are still unknown. Any further investigations to understand the mechanism of regulation of LlaNAC gene expression are likely to find immense importance in plant biotechnology and crop improvement.