Fluorescent X-ray interference from a protein monolayer
Fluorescent X-ray interference from a protein monolayer
复制标题
单层蛋白质的荧光 X 射线干扰
DOI：
10.1126/science.8272866
复制
发表时间：
1994
期刊：
影响因子：
56.9
通讯作者：
T. Ishibashi
中科院分区：
文献类型：
--
作者：
Y. Sasaki;Yoshio Suzuki;T. Ishibashi
荧光x射线干涉模式已被观察到从单分子层的含金属蛋白（铁蛋白）和非金属蛋白[牛血清白蛋白（BSA）]结合在金底物。这些干涉图样已被用来确定结构数据。非金属蛋白质首先通过螯合化合物与金属离子反应，在分子中放置必要的发色团。扫描电镜测得的铁蛋白核的大小与x射线干涉实验的结果一致。在BSA分子中，测量到的干涉条纹符合BSA分子短轴垂直于表面底物的模型。
Fluorescent x-ray interference patterns have been observed from monolayers of both a metal-containing protein (ferritin) and a nonmetal-containing protein [bovine serum albumin (BSA)] bound on a gold substrate. These interference patterns have been used to determine structure data. The nonmetal-containing protein was first reacted with metal ions by means of a chelate compound to place the necessary chromophore in the molecule. The size of the ferritin core measured by a scanning electron microscope agrees with the value obtained from the x-ray interference experiments. In the BSA molecule, the measured interference fringe is consistent with the model in which the short axes of BSA molecules are perpendicular to the surface substrate.