Expression microdissection: operator-independent retrieval of cells for molecular profiling.
Expression microdissection: operator-independent retrieval of cells for molecular profiling.
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表达显微切割：独立于操作员的细胞检索以进行分子分析。
DOI：
10.1097/01.pdm.0000135964.31459.bb
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Emmert-Buck,MichaelR
中科院分区：
文献类型：
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作者：
Tangrea,MichaelA;Chuaqui,RodrigoF;Gillespie,JohnW;Ahram,Mamoun;Gannot,Gallya;Wallis,BenjaminS;Best,CarolynJM;Linehan,WMarston;Liotta,LanceA;Pohida,ThomasJ;Bonner,RobertF;Emmert-Buck,MichaelR
组织显微解剖是研究疾病状态的重要方法。然而，用目前的技术很难进行高通量的分子分析。我们在这里描述了一种新技术(表达显微切割)的原型版本，该技术允许通过分子靶向获得所需的细胞。与现有的方法相比，表达微解剖(XMD)具有显著的优势，包括分离速度提高了几个数量级。XMD可能会成为研究癌症或其他疾病状态的研究人员在患者标本和动物模型中的有价值的工具。
Tissue microdissection is an important method for the study of disease states. However, it is difficult to perform high-throughput molecular analysis with current techniques. We describe here a prototype version of a novel technique (expression microdissection) that allows for the procurement of desired cells via molecular targeting. Expression microdissection (xMD) offers significant advantages over available methods, including an increase in dissection speed of several orders of magnitude. xMD may become a valuable tool for investigators studying cancer or other disease states in patient specimens and animal models.