Dual Role of Wingless Signaling in Stem-like Hematopoietic Precursor Maintenance in Drosophila
Dual Role of Wingless Signaling in Stem-like Hematopoietic Precursor Maintenance in Drosophila
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DOI：
10.1016/j.devcel.2009.03.003
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发表时间：
2009-05-19
影响因子：
11.8
通讯作者：
Banerjee, Utpal
中科院分区：
文献类型：
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作者：
Sinenko, Sergey A.;Mandal, Lolitika;Banerjee, Utpal
在果蝇中，血液发育发生在一个专门的幼虫造血器官，淋巴腺（LG），其中干细胞样血细胞前体或原血细胞分化为多种血细胞类型。在这里，我们表明，无翅（Wg）信号通路的组件在原血细胞中表达。功能丧失和功能获得分析表明，经典Wg信号传导是维持原血细胞所必需的，并负调节其分化。Wg信号在本地以短距离的方式在不同隔室的LG。此外，Wg信号积极调节细胞的增殖和维持功能作为造血生态位在果蝇，后信号中心（PSC），并在晶体细胞的增殖。我们的研究揭示了Wg信号在维持干细胞样血液祖细胞中的保守功能，并揭示了该途径通过其在造血生态位中的作用参与调节血细胞分化。
In Drosophila, blood development occurs in a specialized larval hematopoietic organ, the lymph gland (LG), within which stem-like hemocyte precursors or prohemocytes differentiate to multiple blood cell types. Here we show that components of the Wingless (Wg) signaling pathway are expressed in prohemocytes. Loss- and gain-of-function analysis indicates that canonical Wg signaling is required for maintenance of prohemocytes and negatively regulates their differentiation. Wg signals locally in a short-range fashion within different compartments of the LG. In addition, Wg signaling positively regulates the proliferation and maintenance of cells that function as a hematopoietic niche in Drosophila, the posterior signaling center (PSC), and in the proliferation of crystal cells. Our studies reveal a conserved function of Wg signaling in the maintenance of stem-like blood progenitors and reveal an involvement of this pathway in the regulation of hemocyte differentiation through its action in the hematopoietic niche.