Involvement of the flagellar assembly pathway in Vibrio alginolyticus adhesion under environmental stresses.
Involvement of the flagellar assembly pathway in Vibrio alginolyticus adhesion under environmental stresses.
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环境胁迫下鞭毛组装途径参与溶藻弧菌粘附
DOI：
10.3389/fcimb.2015.00059
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发表时间：
2015
影响因子：
5.7
通讯作者：
Yan Q
中科院分区：
文献类型：
--
作者：
Wang L;Huang L;Su Y;Qin Y;Kong W;Ma Y;Xu X;Lin M;Zheng J;Yan Q
黏附性是溶藻弧菌的重要毒力因子。这一因素可能受环境条件的影响，但其分子机制尚不清楚。在我们以前的研究中，通过在铜、铅、汞和低pH胁迫下培养溶藻弧菌获得了黏附缺陷菌株。通过RNA-SEQ和生物信息学分析，我们发现所有这些胁迫处理都显著影响了鞭毛组装途径，这可能在溶藻弧菌的黏附中发挥重要作用。因此，我们假设鞭毛组装途径的环境压力可能是环境条件影响黏附的一种方式。为了验证我们的假设，我们进行了生物信息学分析、QPCR、RNAi、体外黏附实验和运动实验。我们的结果表明：(1)鞭毛组装途径对环境胁迫敏感，(2)鞭毛组装途径在溶藻弧菌黏附中起重要作用，(3)运动性不是鞭毛组装途径影响黏附的唯一途径。
Adhesion is an important virulence factor of Vibrio alginolyticus. This factor may be affected by environmental conditions; however, its molecular mechanism remains unclear. In our previous research, adhesion deficient strains were obtained by culturing V. alginolyticus under stresses including Cu, Pb, Hg, and low pH. With RNA-seq and bioinformatics analysis, we found that all of these stress treatments significantly affected the flagellar assembly pathway, which may play an important role in V. alginolyticus adhesion. Therefore, we hypothesized that the environmental stresses of the flagellar assembly pathway may be one way in which environmental conditions affect adhesion. To verify our hypothesis, a bioinformatics analysis, QPCR, RNAi, in vitro adhesion assay and motility assay were performed. Our results indicated that (1) the flagellar assembly pathway was sensitive to environmental stresses, (2) the flagellar assembly pathway played an important role in V. alginolyticus adhesion, and (3) motility is not the only way in which the flagellar assembly pathway affects adhesion.