Static Magnetic Fields Inhibit Proliferation and Disperse Subcellular Localization of Gamma Complex Protein3 in Cultured C2C12 Myoblast Cells
Static Magnetic Fields Inhibit Proliferation and Disperse Subcellular Localization of Gamma Complex Protein3 in Cultured C2C12 Myoblast Cells
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DOI：
10.1007/s12013-010-9076-1
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发表时间：
2010-05-01
影响因子：
2.6
通讯作者：
Im, Wooseok
中科院分区：
文献类型：
--
作者：
Kim, SeungChan;Im, Wooseok
磁场可能延缓细胞周期进程的速度，有报道称，磁场诱导培养的神经元细胞的神经突生长。为了验证磁场对成肌细胞生长是否也有影响，我们培养C2C12细胞系，施加2000G静磁场。培养48 h后，WST-1检测（0.01 < P < 0.025， t检验）和直接细胞计数（P < 0.0005， t检验）均显示，静磁场对培养的C2C12细胞增殖有抑制作用。对α和微管蛋白γ复合物蛋白（TUBA和GCP3）进行免疫细胞化学，并应用静态磁场分散微管蛋白GCP3形成，这是细胞分裂过程中微管蛋白结构的细胞内装置。western blot未改变该蛋白的表达。本研究表明，施加静磁场可改变GCP3的亚细胞定位，并可能延缓培养的C2C12成肌细胞的细胞生长。
Magnetic fields may delay the rate of cell cycle progression, and there are reports that magnetic fields induce neurite outgrowth in cultured neuronal cells. To demonstrate whether magnetic field also effects on myoblast cells in cell growth, C2C12 cell lines were cultured and 2000G static magnetic field was applied. After 48 h of incubation, both the WST-1 assay (0.01 < P < 0.025, t-test) and direct cell counting (P < 0.0005, t-test) showed that static magnetic fields inhibit the proliferation of cultured C2C12 cells. Immunocytochemistry for alpha and tubulin gamma complex protein (TUBA and GCP3) was made and applying a static magnetic field-dispersed tubulin GCP3 formation, a intracellular apparatus for tubulin structuring in cell division. This protein expression was not altered by western blot. This study indicates that applying a static magnetic field alters the subcellular localizing of GCP3, and may delay the cell growth in cultured C2C12 myoblast cells.