Isolation of neural stem cells from damaged rat cerebral cortex after traumatic brain injury
Isolation of neural stem cells from damaged rat cerebral cortex after traumatic brain injury
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DOI：
10.1097/01.wnr.0000183330.44112.ab
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发表时间：
2005-10-17
期刊：
影响因子：
1.7
通讯作者：
Ito, H
中科院分区：
文献类型：
--
作者：
Itoh, T;Satou, T;Ito, H
大鼠脑外伤后24 h、72 h和7 d，损伤区周围可见Nestin阳性细胞。在损伤后72 h从损伤区域周围分离组织，并将球与碱性成纤维细胞生长因子和表皮生长因子一起培养。伤后24 h和7 d不能分离出这些球。分离的球体由巢蛋白阳性神经干细胞组成。无碱性成纤维细胞生长因子和表皮生长因子培养4天后，神经球分化为Tujl阳性、胶质细胞酸性蛋白阳性和O4阳性细胞。这些结果表明，分离和培养的神经球可以分化为神经元和胶质细胞。大脑皮质损伤区周围nestin阳性细胞的增加可能有助于神经发生和神经可塑性。
Nestin-positive cells were seen around the damaged area at 24 h, 72 In and 7 days after rat traumatic brain injury. Tissue was isolated from around the damaged area at 72 h after injury and spheres were cultured with basic fibroblast growth factor and epidermal growth factor. These spheres could not be isolated at 24 h and 7 days after injury. Isolated spheres consisted of nestin-positive neural stem cells. Neurospheres differentiated into Tujl-positive, glial fibrillary acidic protein-positive and O4-positive cells after 4 days in culture without basic fibroblast growth factor and epidermal growth factor. These results indicate that isolated and cultured neurospheres can differentiate into neurons and glia. An increase in nestin-positive cells around a cerebral cortical damaged area might contribute to neurogenesis and neuroplasticity.