Antimicrobial susceptibility and molecular epidemiology of Streptococcus pneumoniae isolated from Shanghai, China
Antimicrobial susceptibility and molecular epidemiology of Streptococcus pneumoniae isolated from Shanghai, China
复制标题
DOI：
10.1016/j.ijantimicag.2008.05.004
复制
发表时间：
2008-11-01
影响因子：
10.8
通讯作者：
McGee, Lesley
中科院分区：
文献类型：
--
作者：
Yang, Fan;Xu, Xiao-Gang;McGee, Lesley
本文对2004年和2005年从上海中国呼吸道标本中分离的肺炎球菌的耐药性进行了分析。儿童分离株对青霉素和红霉素的不敏感率分别为63.1%和94.3%，而成人分离株分别为20.5%和69.2%(P<；0.0001和P<；0.0002)。从103株供试菌株中鉴定出19个血清型，其中73.8%属于19F、14、23F、6B和19A。红霉素耐药株中erm(B)基因阳性51株(52.6%)，MEF(E)基因阳性5株(5.2%)，erm(B)和MEF(E)同时阳性41株(42.3%)。通过多位点序列分型(MLST)确定的45个序列类型(STS)中，有25个是新指定的序列类型，9个STS包含10个新等位基因。另一方面，67株青霉素不敏感肺炎链球菌中的46株(68.7%)和97株大环内酯类耐药肺炎链球菌中的51株(52.6%)被鉴定为属于四个国际耐药克隆复合体：台湾(19F)-14、西班牙(23F)-1、西班牙(6B)-2和台湾(23F)-15。我们的研究结果表明，国际抗性无性系的传播对上海地区抗性的出现和增加起到了主导作用。结合疫苗接种可能是预防肺炎球菌耐药性增加的一种有前景的方法。(C)2008年Elsevier B.V.和国际化疗学会。版权所有。
In this study, the antimicrobial resistance profiles of pneumococci isolated from respiratory specimens of patients from Shanghai, China, in 2004 and 2005 are described. Non-susceptible rates to penicillin and erythromycin among paediatric isolates (n = 122) were 63.1% and 94.3%, respectively, whilst those of adult isolates (n = 39) were 20.5% and 69.2%, respectively (P < 0.0001 and P < 0.0002). Nineteen serotypes were identified among 103 tested strains, 73.8% of which belonged to 19F, 14, 23F, 6B and 19A. The erm(B) gene was detected in 51 erythromycin-resistant strains (52.6%), the mef(E) gene in 5 strains (5.2%) and both erm(B) and mef(E) in 41 strains (42.3%). Among 45 sequence types (STs) determined by multilocus sequence typing (MLST) in these 103 isolates, 25 STs were new assignments and 9 STs contained 10 new alleles. On the other hand, 46 (68.7%) of 67 penicillin-non-susceptible S. pneumoniae and 51 ( 52.6%) of 97 macrolide-resistant S. pneumoniae were characterised as belonging to four international resistant clonal complexes, Taiwan(19F)-14, Spain(23F)-1, Spain(6B)-2 and Taiwan(23F)-15. Our findings indicate that the spread of international resistant clones played a predominant role in the emergence and increase of resistance in Shanghai. Conjugate vaccination may be a promising method to prevent the increase in pneumococcal resistance. (C) 2008 Elsevier B. V. and the International Society of Chemotherapy. All rights reserved.