A Novel Little Membrane Protein Confers Salt Tolerance in Rice (Oryza sativa L.)
A Novel Little Membrane Protein Confers Salt Tolerance in Rice (Oryza sativa L.)
复制标题
一种新型小膜蛋白赋予水稻（Oryza sativa L.）耐盐性
DOI：
10.1007/s11105-015-0944-0
复制
发表时间：
2016
影响因子：
2.1
通讯作者：
Huang Ji
中科院分区：
文献类型：
--
作者：
Yuan Xi;Sun Hui;Tang Zhengbin;Tang Haijuan;Zhang Hongsheng;Huang Ji
质膜蛋白在植物感受和响应非生物和生物胁迫中起着重要作用。在本研究中，我们的特点是以前未知的基因应力相关小蛋白1（SALP 1）编码质膜蛋白。SALP 1是一种小的植物特异性膜蛋白，仅含有74个氨基酸残基。SALP 1在水稻不同组织中组成型表达，在根、叶片和幼穗中表达量较高。表达分析表明，SALP 1受到多种非生物胁迫和脱落酸（阿坝）的诱导。亚细胞定位试验表明，SALP 1在水稻原生质体细胞中定位于质膜上。过量表达SALP 1可提高水稻体内游离脯氨酸含量和OsP 5CS基因的表达水平，平衡盐胁迫下的离子含量，从而提高水稻的耐盐性。此外，SALP 1转基因水稻对阿坝处理的敏感性降低，并且SALP 1的表达水平不受ABI 5-like 1蛋白的影响。因此，SALP 1是一种新的膜蛋白，通过ABA非依赖性信号通路参与水稻的耐盐性。
Plasma membrane proteins play critical roles in sensing and responding abiotic and biotic stresses in plants. In the present study, we characterized a previously unknown gene stress associated little protein 1 (SALP1) encoding a plasma membrane protein. SALP1, a small and plant-specific membrane protein, contains only 74 amino acid residues. SALP1 was constitutively expressed in various rice tissues while highly expressed in roots, leaf blade, and immature panicles. Expression analysis indicated that SALP1 was induced by various abiotic stresses and abscisic acid (ABA). Subcellular localization assay indicated that SALP1 was localized on plasma membrane in rice protoplast cells. Overexpressing of SALP1 in rice improved salt tolerance through increasing free proline contents and the expression level of OsP5CS gene, and balancing ion contents under salt stress. Moreover, SALP1 transgenic rice showed reduced sensitivity to ABA treatment, and expression level of SALP1 is not altered by ABI5-like 1 protein. Conclusively, SALP1, a novel membrane protein, is involved in salt tolerance through an ABA-independent signaling pathway in rice.