A comparative proteomic analysis of human and rat embryonic cerebrospinal fluid.
A comparative proteomic analysis of human and rat embryonic cerebrospinal fluid.
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DOI：
10.1021/pr070247w
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发表时间：
2007-08
影响因子：
4.4
通讯作者：
M. Zappaterra;S. Lisgo;S. Lindsay;S. Gygi;C. Walsh;B. Ballif
中科院分区：
文献类型：
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作者：
M. Zappaterra;S. Lisgo;S. Lindsay;S. Gygi;C. Walsh;B. Ballif
在脊椎动物中枢神经系统发育过程中，顶端神经上皮细胞被胚胎脑脊液（e-CSF）所浸润，e-CSF对皮层细胞的增殖和维持起着调节作用。在这里，我们报告的第一个蛋白质组学分析的人e-CSF，并将其与一个广泛的蛋白质组学分析大鼠e-CSF。正如预期的那样，我们在CSF中鉴定了大量蛋白酶抑制剂、细胞外基质蛋白和转运蛋白。然而，我们还发现了一套令人惊讶的信号和细胞内蛋白，这是以前蛋白质组学分析所没有预测到的。一些细胞内蛋白可能代表最近描述的CSF内的微泡的内容物（Marzesco，A. M.，等人，J. Cell Sci. 2005年，118（Pt. 13），2849-2858）。定义e-CSF的丰富组成将使我们能够更好地了解其在大脑发育的关键阶段的协同作用。
During vertebrate central nervous system development, the apical neuroepithelium is bathed with embryonic Cerebrospinal Fluid (e-CSF) which plays regulatory roles in cortical cell proliferation and maintenance. Here, we report the first proteomic analysis of human e-CSF and compare it to an extensive proteomic analysis of rat e-CSF. As expected, we identified a large collection of protease inhibitors, extracellular matrix proteins, and transport proteins in CSF. However, we also found a surprising suite of signaling and intracellular proteins not predicted by previous proteomic analysis. Some of the intracellular proteins are likely to represent the contents of microvesicles recently described within the CSF (Marzesco, A. M., et al. J. Cell Sci. 2005, 118 (Pt. 13), 2849-2858). Defining the rich composition of e-CSF will enable a greater understanding of its concerted actions during critical stages of brain development.