A BAC contig map of the Ly49 gene cluster in 129 mice reveals extensive differences in gene content relative to C57BL/6 mice.
A BAC contig map of the Ly49 gene cluster in 129 mice reveals extensive differences in gene content relative to C57BL/6 mice.
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129 只小鼠的 Ly49 基因簇的 BAC 重叠群图谱揭示了相对于 C57BL/6 小鼠的基因内容的广泛差异。
DOI：
10.1006/geno.2002.6724
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发表时间：
2002
期刊：
影响因子：
4.4
通讯作者：
Anderson,StephenK
中科院分区：
文献类型：
--
作者：
Makrigiannis,AndrewP;Pau,AmandaT;Schwartzberg,PamelaL;McVicar,DanielW;Beck,ThomasW;Anderson,StephenK
鼠Ly 49基因家族在功能上类似于I类主要组织相容性复合体（MHC）受体的人杀伤细胞Ig样受体（KIR）家族。KIR基因的数量在个体之间变化很大;然而，Ly 49基因的组织仅在C57 BL/6（B6）小鼠中确定。129个Ly 49基因座的组织是通过PCR和Southern印迹分析从BAC重叠群图谱确定的。除了从129/J菌株的先前研究中已知的10个Ly 49基因之外，8个新基因定位于129 Ly 49簇。确定Ly 49 q1、e、（v，q2）、e/c2、l/r、s、t、e/c1、r、u、u/i、i1、g、p/d、（i2，p）和o的基因顺序。129个Ly 49基因簇预计跨度为600 kb。这些结果表明，Ly 49基因的数量可以显着不同的近交系小鼠品系，类似于在人类KIR基因中观察到的单倍型差异。
The murine Ly49 gene family is functionally analogous to the human killer cell Ig-like receptor (KIR) family of class I major histocompatibility complex (MHC) receptors. The number of KIR genes varies dramatically between individuals; however, the organization of the Ly49 genes has only been determined for the C57BL/6 (B6) mouse. The organization of the 129 Ly49 loci was determined from a BAC contig map by PCR and Southern blot analysis. In addition to the 10 Ly49 genes known from previous studies of the 129/J strain, 8 new genes were localized to the 129 Ly49 cluster. A gene order of Ly49q1, e, (v, q2), e/c2, l/r, s, t, e/c1, r, u, u/i, i1, g, p/d, (i2, p), and o was determined. The 129 Ly49 gene cluster is predicted to span ∼600 kb. These results indicate that Ly49 gene numbers can be significantly different between inbred mouse strains, analogous to the haplotype differences observed in the human KIR genes.