Co-existence of heparin-binding epidermal growth factorlike growth factor and pinopodes in human endometrium at the time of implantation
Co-existence of heparin-binding epidermal growth factorlike growth factor and pinopodes in human endometrium at the time of implantation
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DOI：
10.1093/molehr/8.8.765
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发表时间：
2002-08-01
影响因子：
4
通讯作者：
Hovatta, O
中科院分区：
文献类型：
--
作者：
Stavreus-Evers, A;Aghajanova, L;Hovatta, O
胞饮突被认为是子宫容受性的标志。肝素结合表皮生长因子样生长因子（HB-EGF）在分泌期子宫内膜中表达量增加，被认为对人类着床过程很重要。本研究的目的是探讨胞饮突和HB-EGF在正常人子宫内膜中可能共存。子宫内膜活检标本取自月经周期正常的妇女。通过扫描电子显微镜检查活检组织中的胞饮突，通过免疫组化检测HB-EGF蛋白的表达，并通过共聚焦显微镜检查，以确定HB-EGF是否存在于胞饮突的表面上。HB-EGF在腔上皮和腺上皮中的表达最高时，充分发育的胞饮突。使用共聚焦显微镜，它表明，HB-EGF存在于管腔上皮细胞内和胞饮突表面。这些发现表明，HB-EGF可能在人类植入过程中的附着和渗透步骤中发挥作用。免疫组化染色结果表明，HB-EGF可作为种植窗的标志物。
Pinopodes have been suggested to be markers of uterine receptivity. Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is expressed in increasing amounts in the secretory phase endometrium and is considered to be important for the human implantation process. The aim of this study was to investigate a possible co-existence of pinopodes and HB-EGF in the normal human endometrium. Endometrial biopsies were obtained from women with normal menstrual cycles. The biopsies were examined by scanning electron microscopy for the detection of pinopodes, by immunohistochemistry for the expression of HB-EGF protein, and by confocal microscopy to determine if HB-EGF was present on the surface of the pinopodes. The expression of HB-EGF in luminal and glandular epithelium was highest when fully developed pinopodes were present. Using confocal microscopy it was shown that HB-EGF was present both inside the luminal epithelial cells and on the surface of pinopodes. These findings suggest that HB-EGF might play a role in both the attachment and penetration steps in the human implantation process. Furthermore, the immunohistochemical staining demonstrates that HB-EGF can be used as a marker for the implantation window.