Methyltransferase-directed DNA strand scission
Methyltransferase-directed DNA strand scission
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DOI：
10.1021/ja054128y
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发表时间：
2005-10-19
影响因子：
15
通讯作者：
Rajski, SR
中科院分区：
文献类型：
--
作者：
Comstock, LR;Rajski, SR
我们在这里证明了mase修饰的DNA可以与菲罗啉衍生的三芳基膦进行Staudinger连接。这些双链体与Cu（II）和3-巯基丙酸的结合会导致MTase识别位点附近的链断裂。M.TaqI和M.HhaI利用合成的叠氮化物辅助因子，产生DNA损伤，诱导被酶修饰的碱基发生5′断裂。这种化学反应代表了一种新的方法，通过这种方法可以根据DNA损伤快速识别DNA甲基化区域。
We demonstrate here that MTase-modified DNA can undergo the Staudinger ligation with triarylphosphines derivatized with phenanthroline. Presentation of these duplexes with Cu(II) and 3-mercaptopropionic acid leads to strand scission proximal to the MTase recognition site. By virtue of their ability to use a synthetic azide-bearing cofactor, M.TaqI and M.HhaI produce a DNA lesion that induces scission 5‘ to the base modified by the enzyme. This chemistry represents a new approach by which regions of DNA methylation can be rapidly identified on the basis of DNA damage.