An origin unwinding activity regulates initiation of DNA replication during mammalian cell cycle.
An origin unwinding activity regulates initiation of DNA replication during mammalian cell cycle.
复制标题
起始解旋活性调节哺乳动物细胞周期中 DNA 复制的起始。
DOI：
10.1126/science.2843984
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发表时间：
1988
期刊：
影响因子：
56.9
通讯作者：
G. D'Urso
中科院分区：
文献类型：
--
作者：
J. Roberts;G. D'Urso
开发了一种体外试验来研究在哺乳动物细胞周期期间调节DNA复制开始的正因子。从细胞周期中确定位置的细胞制备的提取物用于检查无细胞系统中SV40 DNA的复制。从S期细胞制备的提取物在SV40起点启动复制的效率是从G1细胞提取物的10倍，而在G1和S反应中延伸率相似。在细胞周期中的一个离散点，就在细胞进入S之前，出现了一种活性，这种活性是与SV40 T抗原一起在SV40起源处进行位点特异性起始所必需的。该因子在复制起点的DNA解旋中起作用。
An in vitro assay was developed to study the positive factors that regulate the onset of DNA replication during the mammalian cell cycle. Extracts prepared from cells at defined positions in the cell cycle were used to examine the replication of SV40 DNA in a cell free system. Extracts prepared from S phase cells were ten times more efficient at initiating replication at the SV40 origin than were extracts from G1 cells, whereas elongation rates were similar in G1 and S reactions. At a discrete point in the cell cycle, just before the cell's entry into S, an activity appeared that was required, in conjunction with SV40 T antigen, for site specific initiation at the SV40 origin. This factor had a role in unwinding DNA at the replication origin.