STUDIES OF COMPOSITION AND STRUCTURE OF SERUM LIPOPROTEINS - ISOLATION PURIFICATION AND CHARACTERIZATION OF VERY LOW DENSITY LIPOPROTEINS OF A HUMAN SERUM
STUDIES OF COMPOSITION AND STRUCTURE OF SERUM LIPOPROTEINS - ISOLATION PURIFICATION AND CHARACTERIZATION OF VERY LOW DENSITY LIPOPROTEINS OF A HUMAN SERUM
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DOI：
10.1021/bi00879a033
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发表时间：
1965-01-01
期刊：
影响因子：
2.9
通讯作者：
FURMAN, RH
中科院分区：
文献类型：
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作者：
GUSTAFSON, A;ALAUPOVIC, P;FURMAN, RH
与较高密度的α-和β-脂蛋白相比，对极低密度（VLD）* 1脂蛋白的组成和结构知之甚少。虽然脂质部分已被定性，但有关相应蛋白质部分的性质的信息很少。氨基酸分析表明有多达四种不同的蛋白质（Rodbell，1958），而免疫学研究表明只有一种或两种（Marsh和Whereat，1959）。肝素在钙或镁离子存在下沉淀VLD-脂蛋白和β-脂蛋白（Burstein和Samaille 1955，1958），而5%聚乙烯吡咯烷酮（PVP）仅絮凝VLD-脂蛋白（Burstein和Prawerman，1958; Gordis，1962）。各种电泳方法分离VLD-脂蛋白
Irrelatively little is known about the composition and structure of very low density (VLD)* 1 lipoproteins, in comparison with the higher density a- and/3-lipoproteins. Although the lipid moieties have been well characterized qualitatively, there is a paucity of infor-mation concerningthe nature of the corresponding protein moieties. Amino acid analyses have indicated as many as four distinctproteins (Rodbell, 1958), while immunological studies suggest only one or two (Marsh and Whereat, 1959).Very low density serum lipoproteins can be isolated by precipitationwith natural or synthetic polymers. Heparin, in the presence of calcium or magnesium ions precipitates VLD-lipoproteins and/3-lipoproteins (Bur-stein and Samaille 1955, 1958), whereas 5% polyvinylpyrrolidone (PVP) flocculates only VLD-lipoproteins (Burstein and Prawerman, 1958; Gordis, 1962). Separa-tion of VLD-lipoproteins by various electrophoretic